{"code":"SASDC83","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.","publication":{"title":"Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution.","author_list":"Shih O, Yeh YQ, Liao KF, Sung TC, Chiang YW, Jeng US","journal":"Phys Chem Chem Phys","doi":"10.1039/c6cp08820a","pmid":"28262878","published_date":"2017 Mar 15"},"status":"released","submitted_date":"2017-05-03","released_date":"2017-05-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC83.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC83.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC83_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC83_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC83.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 1M-F","resolution":172.0},"name":"Taiwan Photon Source, NSRRC","city":"Hsinchu","country":"Taiwan","beamline_name":"23A","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Apoptosis regulator BAX (Bcl-2 associated X)","short_name":"BAX","sequence":"MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMI\r\nAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG","organism":"Homo sapiens","uniprot_code":"Q07812","uniprot_range_first":1,"uniprot_range_last":192,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS\nECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL\nVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV\nLTASLTIWKKMG","mw":21.184,"total_mw":42.368,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20mM sodium phosphate 100mM NaCl","concentration_unit":null,"comment":"20mM sodium phosphate, 100mM NaCl","additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Dimeric apoptosis regulator BAX (Bcl-2 associated X)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Hsinchu City, Taiwan","full_name":"National Synchrotron Radiation Research Center","webpage":"https://www.nsrrc.org.tw/"}],"contributor_name":"Orion","contributor_surname":"Shih","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-05-28","storage_temperature":null,"cell_temperature":15.0,"exposure_time":30.0,"number_of_frames":3,"wavelength":0.08266,"sample_detector_distance":3.17,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.144,"s_max":2.991,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC83_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC83_fit1_model1.pdb","model_mw":44.72,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":216}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC83_fit1_fit_img.png","software":"CORAL","chi_square_value":1.25,"p_value":0.565228,"fit_residual_plot":"SASDC83_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC83_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/ad28.log","software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of dimeric apoptosis regulator BAX (Bcl-2 associated X) in 20mM sodium phosphate, 100mM NaCl, pH 8 were collected on the 23A beam line at the Taiwan Photon Source (NSRRC, Hsinchu, Taiwan) using a Pilatus 1M-F detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.08266 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 15°C. Three successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to obtain the SAXS profile displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":39.5,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.01585,"pddf_i0_error":0.000135,"guinier_i0":0.01578,"guinier_i0_error":0.0002,"pddf_rg":3.01,"pddf_rg_error":0.03,"guinier_rg":2.97,"guinier_rg_error":0.06,"pddf_dmax":9.52,"pddf_dmax_error":null,"porod_volume":85.57,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":38,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC83_datloglog_img.png","symmetry":null,"last_modified":"2021-12-03T19:22:36.894202+01:00","bragg_peak":[]}