{"code":"SASDC85","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities.","publication":{"title":"Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities.","author_list":"Batista FA, Almeida GS, Seraphim TV, Silva KP, Murta SM, Barbosa LR, Borges JC","journal":"FEBS J","doi":"10.1111/febs.13141","pmid":"25369258","published_date":"2015 Jan"},"status":"released","submitted_date":"2017-06-17","released_date":"2017-06-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC85.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC85.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC85_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC85_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC85.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS2 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Leishmania braziliensis p23 isoform B","short_name":"Lbp23B","sequence":"GSHMSASGSLVPPISWAQRPEYVLVTIPLQDTTGVTVEIKDEGRELLFACCAPEGKQYACTIHFYGAISS\r\nEESQHVVRPRQIELKLRKKLTRSLEDADDEVEWPRLTKEKVKYPNITIDWSKWKDEDDEGATDDLGDFGL\r\nGGGDAMDGQYSEMLSQLMQTQGKKDAEELAGLPPGTIPEFGSAQRQEATHGASAAAGDDDSEMPPLEDDM","organism":"Leishmania braziliensis","uniprot_code":"A4HAD9","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSASGSLVPPISWAQRPEYVLVTIPLQDTTGVTVEIKDEGRELLFACCAPEGKQYACTIH\nFYGAISSEESQHVVRPRQIELKLRKKLTRSLEDADDEVEWPRLTKEKVKYPNITIDWSKW\nKDEDDEGATDDLGDFGLGGGDAMDGQYSEMLSQLMQTQGKKDAEELAGLPPGTIPEFGSA\nQRQEATHGASAAAGDDDSEMPPLEDDM","mw":23.045,"total_mw":23.045,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Leishmania braziliensis p23B","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-03-26","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":300.0,"number_of_frames":null,"wavelength":0.1488,"sample_detector_distance":1.0,"concentration_min":1.0,"concentration_max":4.0,"sample_volume":null,"flow_rate":null,"s_min":0.162,"s_max":3.346,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDC85_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5.0","model_data":"https://www.sasbdb.org/media/pdb_file/SASDC85_fit1_model1.pdb","model_mw":34.41,"bead_radius":null,"log":null,"symmetry":"P1","comment":"","user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDC85_fit1_fit_img.png","software":"DAMMIN","chi_square_value":2.83,"p_value":5.4e-05,"fit_residual_plot":"SASDC85_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDC85_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/Lbp23B5.log","software_version":"5.3","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS experiments were performed at the SAXS2 beam line in the Laboratório Nacional de Luz Síncrotron (LNLS, Campinas-SP, Brazil). The X-ray scattering data (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle; λ = 0.1488 nm) were acquired using a two-dimensional MAR-CCD detector. Measurements were performed with a monochromatic X-ray beam and a sample-to-detector distance of ~1000 mm, corresponding to a scattering vector range of 0.015 < q < 0.35 Å−1. Scattering patterns were recorded at different sample concentrations (approximately 1.0, 2.0 and 4.0 mg/mL) and several frames in order to check for protein aggregation and X-ray damage. No evidence of aggregation or inter-particle interference were observed. The model depicts the averaged spatial representation of the protein (DAMFILT occupancy and volume-corrected bead model).","tags":[],"intensity_unit":null,"experimental_mw":26.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.02014,"pddf_i0_error":0.0004,"guinier_i0":0.0200666,"guinier_i0_error":0.00014,"pddf_rg":3.17,"pddf_rg_error":0.2,"guinier_rg":3.03,"guinier_rg_error":0.07,"pddf_dmax":13.0,"pddf_dmax_error":5.0,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":10,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC85_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}