{"code":"SASDC99","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.","publication":{"title":"Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles.","author_list":"Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P","journal":"Protein Sci","doi":"10.1002/pro.3365","pmid":"29266475","published_date":"2018 Mar"},"status":"released","submitted_date":"2017-11-21","released_date":"2018-01-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDC99.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDC99.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC99_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC99_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDC99_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDC99_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDC99.sascif","experiment":{"instrument":{"detector":{"type":"640x640 mm 3He posn sensitive prop counter","name":"Ordela 2660N","resolution":5.08},"name":"NIST Center for High Resolution Neutron Scattering (CHRNS)","city":"Gaithersburg, MD","country":"USA","beamline_name":"NG7","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Apolipoprotein A-I","short_name":"ApoA-I","sequence":"MHHHHHHGENLYFQGMLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTKAQSVIDKASETLTAQ","organism":"Mus musculus","uniprot_code":"Q00623","uniprot_range_first":67,"uniprot_range_last":264,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MKAVVLAVALVFLTGSQAWHVWQQDEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSS\nLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKV\nQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVD\nSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLM\nPMLETLKTQVQSVIDKASETLTAQ","mw":25.153,"total_mw":50.306,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"1,2-dimyristoyl-sn-glycero-3-phosphocholine","short_name":"DMPC","sequence":"N/A","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"other","molecular_type":"other","uniprot_sequence":null,"mw":0.678,"total_mw":92.0,"number_molecules":0,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"PBS in D2O","concentration_unit":null,"comment":"8 g/L NaCl, 0.2 g/L KH2PO4, 1.15 g/L Na2HPO4, 0.2 g/L KCl, in D2O with no additional pH adjustment","additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Nanolipoprotein Particle (NLP) from In Vitro Assembly - 100% D2O SANS","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"NIST","address":"Gaithersburg, MD, USA","full_name":"National Institute of Standards and Technology (NIST)","webpage":"https://www.nist.gov/"}],"contributor_name":"Thomas","contributor_surname":"Cleveland","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-11-25","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1800.0,"number_of_frames":2,"wavelength":0.6,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":5.21,"sample_volume":null,"flow_rate":null,"s_min":0.039,"s_max":5.936,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Additional SANS measurement parameters. Three detector positions (in m) and count times (s) were employed: 1 m (two frames of 300 + 900 = 1200 s total count time); 4 m (two frames of 1800 + 1800 = 3600 s total count time); 13 m (two frames of 300 + 600 = 900 s total count time).","experiment_description":"Small-angle neutron scattering (SANS) data from solutions of Nanolipoprotein Particle (NLP) from In Vitro Assembly in PBS constituted in 100% D2O, pD 7.4, were collected on the NG7 SANS instrument at the NIST Center for Neutron Research (Gaithersburg, MD, USA) using a 640x640 mm 3He position sensitive Ordela 2660N detector at wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.21 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":142.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":2.621,"pddf_i0_error":0.006,"guinier_i0":2.6,"guinier_i0_error":0.0046,"pddf_rg":3.121,"pddf_rg_error":0.007,"guinier_rg":3.11,"guinier_rg_error":0.06,"pddf_dmax":9.09,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":86,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDC99_datloglog_img.png","symmetry":null,"last_modified":"2022-08-15T15:12:53.046326+02:00","bragg_peak":[]}