{"code":"SASDCC7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.","publication":{"title":"Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.","author_list":"Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G","journal":"Structure","doi":"10.1016/j.str.2017.10.001","pmid":"29107484","published_date":"2017 Dec 5"},"status":"released","submitted_date":"2017-09-01","released_date":"2017-10-31"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCC7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCC7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCC7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCC7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"T.maritima PDE","short_name":"TmPDE D80N D154N","sequence":"GSGSGMDEIVKVLSQHDRILVVGHIMPDGDCVSSVLSLTLGLEKLGKEVKAAVDYKIPYVFEKFPYIDKIEENPNFDPELLVVVNASSPDRIGKFQDLLDKVPSVVIDHHSTNTNFGNWNWVDPSFAATAQMIFRINKALGVEYDSNLATLNYLGIATNTGFFRHSNADVRVFEDAYKLVKMGADAHFVAKEILENKRFEQFKLFAEVLERLQLLENGKIAYSYIDYDTYLRHNCTDEDSAGFVGELRSIRGVEVAVLFMEFPRGKIHVSMRSKDWFNVNEVAFELGGGGHPRAAGVTFEGKKIEEVIPRVINHLLKKFKEGVESESEKIPEGDVLGG","organism":"Thermotoga maritima","uniprot_code":"Q9X1T1","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MDEIVKVLSQHDRILVVGHIMPDGDCVSSVLSLTLGLEKLGKEVKAAVDYKIPYVFEKFP\nYIDKIEENPNFDPELLVVVDASSPDRIGKFQDLLDKVPSVVIDHHSTNTNFGNWNWVDPS\nFAATAQMIFRINKALGVEYDSNLATLNYLGIATDTGFFRHSNADVRVFEDAYKLVKMGAD\nAHFVAKEILENKRFEQFKLFAEVLERLQLLENGKIAYSYIDYDTYLRHNCTDEDSAGFVG\nELRSIRGVEVAVLFMEFPRGKIHVSMRSKDWFNVNEVAFELGGGGHPRAAGVTFEGKKIE\nEVIPRVINHLLKKFKEGVESESEKIPEGDVLGG","mw":37.821,"total_mw":75.642,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Munich, Germany","full_name":"Ludwig-Maximilians-Universität München","webpage":"https://www.en.uni-muenchen.de"}],"contributor_name":"Gregor","contributor_surname":"Witte","orcid":"https://orcid.org/0000-0003-0313-0962"}],"concentration_method":null,"concentration_unit":null,"date":"2016-06-17","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.995,"number_of_frames":null,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.0,"s_max":2.927,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant) in 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH 8, were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Samples were collected using SEC-SAXS at 20°C. The scattering was obtained by averaging the main peak's scattering data of a SECSAXS run with TmPDE D80ND154N on a 10/300 S200 superdex column. The data was corrected for buffer scattering by subtraction of an averaged buffer region using the CHROMIXS software (ATSAS 2.8.1).","tags":[],"intensity_unit":"arbitrary","experimental_mw":72.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":69.2,"porod_mw_error":null,"pddf_i0":9314.0,"pddf_i0_error":null,"guinier_i0":9293.71,"guinier_i0_error":6.68,"pddf_rg":2.78,"pddf_rg_error":null,"guinier_rg":2.79,"guinier_rg_error":0.03,"pddf_dmax":7.9,"pddf_dmax_error":null,"porod_volume":115.38,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":54,"guinier_point_last":175,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCC7_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}