{"code":"SASDCH8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"WAXS benchmark on standard proteins","publication":null,"status":"released","submitted_date":"2017-10-17","released_date":"2021-09-13"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCH8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCH8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCH8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCH8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCH8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cytochrome c","short_name":null,"sequence":"GDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKM\r\nIFAGIKKKTEREDLIAYLKKATNE","organism":"Equus caballus","uniprot_code":"P00004","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITW\nKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE","mw":11.701,"total_mw":11.701,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"tbs","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Horse heart cytochrome C (WAXS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Maxim","contributor_surname":"Petoukhov","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-07-16","storage_temperature":10.0,"cell_temperature":20.0,"exposure_time":0.1,"number_of_frames":34,"wavelength":0.062,"sample_detector_distance":1.5,"concentration_min":null,"concentration_max":2.6,"sample_volume":null,"flow_rate":null,"s_min":0.087,"s_max":17.929,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCH8_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"1hrc","ext_code":"1HRC","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCH8_fit1_model1.pdb","model_mw":12.37,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":102}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCH8_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":19.492,"p_value":0.0,"fit_residual_plot":"SASDCH8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCH8_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/1hrc04.log","software_version":"crysol 3.0","description":null}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of horse heart cytochrome C in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring  (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml was measured at 20°C. 34 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the scaled to protein concentration.","tags":["benchmark"],"intensity_unit":"arbitrary","experimental_mw":7.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":11681.1,"guinier_i0_error":8.07,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":1.21,"guinier_rg_error":0.01,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":8,"guinier_point_last":83,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCH8_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}