{"code":"SASDCL3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.","publication":{"title":"Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.","author_list":"Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH","journal":"Structure","doi":"10.1016/j.str.2015.02.012","pmid":"25817386","published_date":"2015 May 5"},"status":"released","submitted_date":"2017-05-18","released_date":"2017-05-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCL3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCL3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCL3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Serine/threonine-protein kinase ATG1","short_name":"Atg1","sequence":"DSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDYDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQ\r\nTLTSATTKISSATNVDTQIPAPELKKLCTESLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQ\r\nWIRDRFNECLDKAEFLRLKLHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCEL\r\nAYATSLWMLEILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV","organism":"Kluyveromyces lactis","uniprot_code":"Q6CSX2","uniprot_range_first":562,"uniprot_range_last":831,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSSESHDKVVAKAIRLPTENYSVEKEIGKGSFAVVYKGLSLRDGRNIAIKAVSRSKLKNK\nKLLENLEVEIAILKKIKHPHIVGLIDCERTSSDFYLIMEYCALGDLTFFIKKRKNLVLKH\nPLIKTVFEHYPPPSTEHNGLNRVLVVNYLQQLSSALKFLRSKNLVHRDIKPQNLLLCTPL\nLDYNDPKTFHELGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKY\nNAKADLWSVGTVLYEMCCGRPPFKASNHLELFQKIKKANDEITVPSNCYIEPKLFNLIRG\nLLTFDPDSRMGFTDFFNNEVVTEDLTRYEQSYEPDLESKSKDVAESNMFVSEYLVKPLKQ\nQESAHIPPTQTDENTSVQTGVRRTSGKERLATNHPPHQQIHPEDNSQNPEQSYQSASQKR\nLKSSYNDLILEKEYVVVEKKTVEVNSLADDFANNGPITNNQGAQVIKPLRYRTSSSSDAS\nGGRRASLVERRLSISSLSPSNALSKALGLASVRLFGYQHNTKATSSPPQQTLLNPQIFQE\nLTENAVLRADHKLNPFSEQMLDSNITPAVESLAAKAFVMYSFAEMKFSQILPTPPSSTDY\nDPLSDKRLSNGSCAIEDEEDLDQGRPPSNQTLTSATTKISSATNVDTQIPAPELKKLCTE\nSLLLYLKALTILAASMKLTSKWWYENESKNCTLKLNILVQWIRDRFNECLDKAEFLRLKL\nHAINTSPNSQWSDDDPVIFVEKLIYDRALDISRNAARMEMESGNYNTCELAYATSLWMLE\nILLDENFQFNEVYDDEYASNITSLDESDKEMIKKYISSIANRLKALKSKMV","mw":30.664,"total_mw":61.328,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Autophagy-related protein 13","short_name":"Atg13","sequence":"FDRTAKPRKSTENPPEDLLEF\r\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNV","organism":"Kluyveromyces lactis","uniprot_code":"Q6CWK2","uniprot_range_first":400,"uniprot_range_last":475,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSEGEVQVIELINNFFLKSALLLEQSKVAHNFDTEEALRDGNHLFNIETRGDPLLEAQIQ\nPWITFDGVKTMPPLVIETYLDLRALQPNHMVYLHDADGNPWMVCKGGKKTEIVLERWLVE\nLDKQTIDDSIDSNDPENLHKQLVLLFRYLYTLTQLLPANDIITKPHNSQQPALINVQTRL\nLDGSKPILSKGRVGLSKPIIASYSNTMNETNIASHLEQRKITPIKTTFGSLRITVSYRKD\nVDFYVIDSDDLQKRYMTPSLSNETTTVPDRRASSNCSRSMSVSPKTNTINATLFPLEGSS\nARRQSISSKLQPFKVGSVGSGSFVQSGSAQSTTSLVPSLSRNVSSSSVVAALKVQRGSAG\nSTIVNGDVPPELSSVGSGSKYSSSFGRIRRHSSIRRSESFDRTAKPRKSTENPPEDLLEF\nVKLLEDKKELNMKPNTILPQQDISNSLMRFQSMKSNNDALSDNLSMSMSIDQPNVRFGSN\nSHSPIPSFSPNYGSIPSRLSQGSRNNSNAELITSRKSSLDRHKHNLLSRTGSNVDINRRG\nSVGTMETTNEDSKEDEQSHMKGMHFSNEATINKDDDEDEMLMKRSFNAGTSTTEQVMGSP\nRSIRSISVSSYPRNQLPLKHLNLSHPTTSATTTHAKFHKSEMSPDPLHTEGAQPHTSSQH\nHNSSQKNDEDDDLLFVMSDMNLTN","mw":8.726,"total_mw":17.452,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 200 mM NaCl, 2% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Atg1-Atg13 Subcomplex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Hanover, NH, USA","full_name":"Dartmouth College","webpage":"http://home.dartmouth.edu/"}],"contributor_name":"Michael","contributor_surname":"Ragusa","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-12-10","storage_temperature":null,"cell_temperature":22.0,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":1.0,"concentration_max":5.2,"sample_volume":null,"flow_rate":null,"s_min":0.089,"s_max":2.719,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the Atg1-Atg13 Subcomplex in 20 mM Tris, 200 mM NaCl, 2% glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5.2 mg/ml were measured at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":null,"experimental_mw":78.5,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1013.0,"pddf_i0_error":2.2,"guinier_i0":1019.45,"guinier_i0_error":2.2,"pddf_rg":3.3,"pddf_rg_error":null,"guinier_rg":3.32,"guinier_rg_error":0.2,"pddf_dmax":10.9,"pddf_dmax_error":null,"porod_volume":160.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":60,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCL3_datloglog_img.png","symmetry":null,"last_modified":"2020-11-24T17:34:01.079622+01:00","bragg_peak":[]}