{"code":"SASDCL6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.","publication":{"title":"Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition.","author_list":"Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C","journal":"Elife","doi":"10.7554/eLife.05767","pmid":"26090905","published_date":"2015 Jun 19"},"status":"released","submitted_date":"2017-07-18","released_date":"2017-07-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCL6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCL6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCL6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCL6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCL6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Shanghai Synchrotron Radiation Facility (SSRF)","city":"Shanghai","country":"China","beamline_name":"BL19U2","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Polyubiquitin-C","short_name":"ubiquitin","sequence":"MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG","organism":"Homo sapiens","uniprot_code":"P0CG48","uniprot_range_first":305,"uniprot_range_last":379,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN\nIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI\nFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA\nKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT\nITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR\nLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL\nSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ\nQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE\nNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL\nTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH\nLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED\nGRTLSDYNIQKESTLHLVLRLRGGV","mw":8.565,"total_mw":17.13,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"100mM NaCl, 10mM sodium acetate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.0,"deuteration":null},"purity_method":null,"name":"Lys63-linked dimer ubiquitin","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Wuhan, China","full_name":"Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences","webpage":"http://english.wipm.cas.cn/"}],"contributor_name":"Zhu","contributor_surname":"Liuzhu","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-03-24","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":20,"wavelength":0.1033,"sample_detector_distance":2.126,"concentration_min":null,"concentration_max":5.1,"sample_volume":null,"flow_rate":null,"s_min":0.123,"s_max":4.699,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of 0.3mM K63-diUb in 100mM NaCl, 10mM sodium acetate, pH 6 were collected on the BL19U2 camera on the storage ring Shanghai Synchrotron Radiation Facility (SSRF) (Shanghai, China). Using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm one solute concentration of 5.1 mg/ml was measured at 25°C. 20 successive 1 second frames were collected, the data were normalized to the intensity of the transmitted beam and radially averaged, the scattering of the solvent-blank was subtracted. The angular range between 0.271 and 2.282 1/nm was used for PDDF conversion.","tags":[],"intensity_unit":null,"experimental_mw":14.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":14.0,"porod_mw_error":null,"pddf_i0":69.0,"pddf_i0_error":0.07,"guinier_i0":69.0664,"guinier_i0_error":0.01,"pddf_rg":2.115,"pddf_rg_error":0.024,"guinier_rg":2.084,"guinier_rg_error":0.01,"pddf_dmax":6.5,"pddf_dmax_error":null,"porod_volume":23.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":31,"guinier_point_last":101,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCL6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}