{"code":"SASDCM3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.","publication":{"title":"Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.","author_list":"Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH","journal":"Structure","doi":"10.1016/j.str.2015.02.012","pmid":"25817386","published_date":"2015 May 5"},"status":"released","submitted_date":"2017-05-18","released_date":"2017-05-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCM3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCM3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCM3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCM3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Autophagy-related protein 17","short_name":"Atg17","sequence":"MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILNCLVNQVEFFK\r\nNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDLTDDHSSLSDFICMDHVDILQ\r\nQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLRTYFDSHFSRDSKNNLFKLLESDYVTELNEF\r\nENELADFLRSITDHFDKCTILKEQQIPLPDLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETI\r\nSEIMDKIGGFHLLASKIVTELEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLL\r\nRERDRRKATALQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\r\nVPN","organism":"Kluyveromyces lactis","uniprot_code":"Q6CS99","uniprot_range_first":1,"uniprot_range_last":423,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSDAATTIPSDEIDKLWNKARNQLVEAQVECEESLKILSKVRTEMDSSQKSRFKLKFILN\nCLVNQVEFFKNIMLEKCISTELIDNEWSKLVLVEIVNDVSYWQNEITTKMKILQGTKYDL\nTDDHSSLSDFICMDHVDILQQKIDEIPIIKQQVTNIRQHYKSIKDRIENQLVAVKLKKLR\nTYFDSHFSRDSKNNLFKLLESDYVTELNEFENELADFLRSITDHFDKCTILKEQQIPLPD\nLKELFQIVKKDDTQLENIRELVFETGLEVKAFSKKVNETISEIMDKIGGFHLLASKIVTE\nLEKCEEYLSIFQKIANLVFVYKESCIKKIEQVQQLCEFYDKFKLGYKNLLRERDRRKATA\nLQMEKILKECQEKLQALSDADLDQRQQFLLENGDYLPENIWPGYIDDMESMYSFEYSIHN\nVPN","mw":49.864,"total_mw":99.728,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Autophagy-related protein 29","short_name":null,"sequence":"MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGAPEPFLKKRSY\r\nTLFQNQLKVLSNQID","organism":"Kluyveromyces lactis","uniprot_code":"Q6CTU8","uniprot_range_first":1,"uniprot_range_last":85,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MNNDNTKVYVRVAGKRPENFVETKPFIWDSRRDKILWTKISKIDSLEDMDWQELGADLGA\nPEPFLKKRSYTLFQNQLKVLSNQIDVTSNTRSSSESRNSVDNNILQNLQASRIMNHKLDK\nTDNLANNQESSSELSNLSVSKSALEDALMDCLQL","mw":10.031,"total_mw":20.062,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"KLLA0A10637p","short_name":"Atg31","sequence":"MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLDYENVIIIDSE\r\nPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGLDNLSKLYHERNAQIRKLLDS\r\nIAP","organism":"Kluyveromyces lactis","uniprot_code":"Q6CX74","uniprot_range_first":1,"uniprot_range_last":143,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MESPVLLVKNANAATNASNLGLLGQKQNSYTVDENTWFLNNISFIFEDDDPSQHDEDQLD\nYENVIIIDSEPSGTVNNVEMLSERWQLLAFDQNPRDNCISLTVMNELSADLGQNTDISGL\nDNLSKLYHERNAQIRKLLDSIAP","mw":16.071,"total_mw":32.142,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 200 mM NaCl, 2% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Atg17-Atg31-Atg29 Subcomplex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Hanover, NH, USA","full_name":"Dartmouth College","webpage":"http://home.dartmouth.edu/"}],"contributor_name":"Michael","contributor_surname":"Ragusa","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-12-10","storage_temperature":null,"cell_temperature":22.0,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":1.0,"concentration_max":5.3,"sample_volume":null,"flow_rate":null,"s_min":0.089,"s_max":2.719,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Atg17-Atg31-Atg29 Subcomplex in 20 mM Tris, 200 mM NaCl, 2% glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5.3 mg/ml were measured at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":null,"experimental_mw":152.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":2368.0,"pddf_i0_error":82.0,"guinier_i0":2325.3,"guinier_i0_error":28.0,"pddf_rg":10.9,"pddf_rg_error":0.2,"guinier_rg":10.1,"guinier_rg_error":0.5,"pddf_dmax":34.0,"pddf_dmax_error":null,"porod_volume":848.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":8,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCM3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}