{"code":"SASDCM8","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.","publication":{"title":"Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.","author_list":"Krausze J, Probst C, Curth U, Reichelt J, Saha S, Schafflick D, Heinz DW, Mendel RR, Kruse T","journal":"Biochem J","doi":"10.1042/BCJ20160846","pmid":"27803248","published_date":"2017 Jan 1"},"status":"released","submitted_date":"2017-10-26","released_date":"2021-02-08"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCM8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCM8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCM8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCM8.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"ID14-3","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Molybdopterin biosynthesis protein CNX1","short_name":"Cnx1E","sequence":"MSRGSMEGQGCCGGGGGKTEMIPTEEALRIVFGVSKRLPPVIVSLYEALGKVLAEDIRAP\r\nDPLPPYPASVKDGYAVVASDGPGEYPVITESRAGNDGLGVTVTPGTVAYVTTGGPIPDGA\r\nDAVVQVEDTKVIGDVSTESKRVKILIQTKKGTDIRRVGCDIEKDATVLTTGERIGASEIG\r\nLLATAGVTMVKVYPMPIVAILSTGDELVEPTAGTLGRGQIRDSNRAMLVAAVMQQQCKVV\r\nDLGIVRDDRKELEKVLDEAVSSGVDIILTSGGVSMGDRDFVKPLLEEKGKVYFSKVLMKP\r\nGKPLTFAEIRAKPTESMLGKTVLAFGLPGNPVSCLVCFNIFVVPTIRQLAGWTSPHPLRV\r\nRLRLQEPIKSDPIRPEFHRAIIKWKDNDGSGTPGFVAESTGHQMSSRLLSMRSANALLEL\r\nPATGNVLSAGSSVSAIIVSDISAFSIDKKASLSEPGSTSGGSAWSHPQFEK","organism":"Arabidopsis thaliana","uniprot_code":"Q39054","uniprot_range_first":1,"uniprot_range_last":451,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MEGQGCCGGGGGKTEMIPTEEALRIVFGVSKRLPPVIVSLYEALGKVLAEDIRAPDPLPP\nYPASVKDGYAVVASDGPGEYPVITESRAGNDGLGVTVTPGTVAYVTTGGPIPDGADAVVQ\nVEDTKVIGDVSTESKRVKILIQTKKGTDIRRVGCDIEKDATVLTTGERIGASEIGLLATA\nGVTMVKVYPMPIVAILSTGDELVEPTAGTLGRGQIRDSNRAMLVAAVMQQQCKVVDLGIV\nRDDRKELEKVLDEAVSSGVDIILTSGGVSMGDRDFVKPLLEEKGKVYFSKVLMKPGKPLT\nFAEIRAKPTESMLGKTVLAFGLPGNPVSCLVCFNIFVVPTIRQLAGWTSPHPLRVRLRLQ\nEPIKSDPIRPEFHRAIIKWKDNDGSGTPGFVAESTGHQMSSRLLSMRSANALLELPATGN\nVLSAGSSVSAIIVSDISAFSIDKKASLSEPGSIRKEKKYDEVPGPEYKVAILTVSDTVSA\nGAGPDRSGPRAVSVVDSSSEKLGGAKVVATAVVPDEVERIKDILQKWSDVDEMDLILTLG\nGTGFTPRDVTPEATKKVIERETPGLLFVMMQESLKITPFAMLSRSAAGIRGSTLIINMPG\nNPNAVAECMEALLPALKHALKQIKGDKREKHPKHIPHAEATLPTDTWDQSYKSAYETGEK\nKEEAGCSCTH","mw":49.7,"total_mw":99.4,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Hepes/KOH, 150 mM NaCl, 1 mM EDTA, 2 % (v/v) glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Mo-insertase domain Cnx1E from Arabidopsis thaliana","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Braunschweig, Germany","full_name":"Braunschweig University of Technology","webpage":"https://www.tu-braunschweig.de/"}],"contributor_name":"Joern","contributor_surname":"Krausze","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-02-05","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.09919,"sample_detector_distance":2.864,"concentration_min":1.65,"concentration_max":12.5,"sample_volume":null,"flow_rate":null,"s_min":0.051,"s_max":4.975,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCM8_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"5g2r","ext_code":"5G2R","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"mix","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCM8_fit1_model1.pdb","model_mw":97.62,"bead_radius":1.9,"log":null,"symmetry":"C2","comment":"C2 symmetry generated from crystallographic symmetry","user":247}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCM8_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":39.748,"p_value":0.0,"fit_residual_plot":"fit4columns1144_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCM8_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/SAXS_AB_maestro_17102600.log","software_version":"2.8.2","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":null,"tags":[],"intensity_unit":"arbitrary","experimental_mw":99.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":105.9,"porod_mw_error":null,"pddf_i0":69.92,"pddf_i0_error":0.029,"guinier_i0":68.97,"guinier_i0_error":0.044,"pddf_rg":3.805,"pddf_rg_error":0.004,"guinier_rg":3.64,"guinier_rg_error":0.06,"pddf_dmax":15.0,"pddf_dmax_error":null,"porod_volume":180.59,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":54,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCM8_datloglog_img.png","symmetry":null,"last_modified":"2021-02-08T20:12:23.492874+01:00","bragg_peak":[]}