{"code":"SASDCP8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The SAXS Analysis of the human GTPase Rab1a","publication":null,"status":"released","submitted_date":"2017-10-29","released_date":"2024-10-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCP8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCP8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCP8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCP8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCP8.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"AVIEX PCCD170170","resolution":null},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ras-related protein Rab-1A","short_name":"Rab1a","sequence":"MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQ\r\nERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAK\r\nEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCCHHHHHH","organism":"Homo sapiens","uniprot_code":"P62820","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTI\nKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKL\nLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGA\nTAGGAEKSNVKIQSTPVKQSGGGCC","mw":23.5,"total_mw":23.5,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Ras-related protein Rab-1A","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Cachan, France","full_name":"CNRS UMR8113, ENS Paris-Saclay","webpage":"http://lbpa.ens-paris-saclay.fr/"}],"contributor_name":"Wenhua","contributor_surname":"Zhang","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-11-17","storage_temperature":null,"cell_temperature":15.0,"exposure_time":1.2,"number_of_frames":240,"wavelength":0.103,"sample_detector_distance":1.2,"concentration_min":2.0,"concentration_max":6.0,"sample_volume":null,"flow_rate":null,"s_min":0.169,"s_max":6.125,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCP8_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.8.2","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCP8_fit1_model1.pdb","model_mw":24.2,"bead_radius":1.4,"log":"https://www.sasbdb.org/media/log_files/Rab1-df.log","symmetry":"P1","comment":"","user":84}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCP8_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.745,"p_value":5e-06,"fit_residual_plot":"SASDCP8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCP8_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/Rab1-df.log","software_version":"2.8.2","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"2.8.2","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Ras-related protein Rab-1A in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5, were collected at the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 2 and 6 mg/ml were measured at 15°C. 240 successive 1.2second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":"1/cm","experimental_mw":24.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":24.0,"porod_mw_error":null,"pddf_i0":0.01385,"pddf_i0_error":1.4e-05,"guinier_i0":0.01366,"guinier_i0_error":1.3e-05,"pddf_rg":2.54,"pddf_rg_error":0.006,"guinier_rg":2.38,"guinier_rg_error":0.004,"pddf_dmax":10.2,"pddf_dmax_error":null,"porod_volume":36.1,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":59,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCP8_datloglog_img.png","symmetry":null,"last_modified":"2024-10-31T15:53:47.550615+01:00","bragg_peak":[]}