{"code":"SASDCQ6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.","publication":{"title":"Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.","author_list":"White MA, Tsalkova T, Mei FC, Cheng X","journal":"Cells","doi":"10.3390/cells9010035","pmid":"31877746","published_date":"2019 Dec 21"},"status":"released","submitted_date":"2017-07-27","released_date":"2019-12-02"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCQ6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCQ6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCQ6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCQ6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Sealy Center For Structural Biology, UTMB-G","city":"Galveston, TX","country":"USA","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Rap guanine nucleotide exchange factor 3","short_name":"EPAC1B","sequence":"GSPGIRVL\r\nVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSES\r\nLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHS\r\nRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVGTHEMEEELA\r\nEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLL\r\nFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA\r\nATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTP\r\nGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHH\r\nFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDT\r\nRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVP\r\nYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVA\r\nTSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSI\r\nHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKL\r\nAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYR\r\nLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPV\r\nPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSREL\r\nEP","organism":"Homo sapiens","uniprot_code":"O95398","uniprot_range_first":2,"uniprot_range_last":881,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLL\nEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRK\nYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQ\nFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEEL\nLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGL\nVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHG\nKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSD\nFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSML\nHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWL\nPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQV\nLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDL\nVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVAT\nELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKV\nRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFE\nKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWA\nYVQQLKVIDNQRELSRLSRELEP","mw":100.001,"total_mw":100.001,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"1mM EDTA, 10mM DTT, 500mM NaCl, and 10mM Tris","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":9.0,"deuteration":null},"purity_method":null,"name":"Rap guanine nucleotide exchange factor 3 (isoform3) - apo form","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Texas, USA","full_name":"University of Texas Medical Branch","webpage":"http://www.bmb.utmb.edu/"}],"contributor_name":"Mark","contributor_surname":"White","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-09-07","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":21600.0,"number_of_frames":12,"wavelength":0.15418,"sample_detector_distance":0.489,"concentration_min":null,"concentration_max":0.5,"sample_volume":null,"flow_rate":null,"s_min":0.108,"s_max":6.753,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCQ6_fit1_model1_img.png","software":"SwissModel","pdb_link":[{"pdb_code":"4f7z","ext_code":"4F7Z","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCQ6_fit1_model1.pdb","model_mw":99.98,"bead_radius":1.9,"log":null,"symmetry":"P1","comment":"","user":85}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCQ6_fit1_fixed_fit_img.png","software":"CORAL","chi_square_value":1.02,"p_value":0.799,"fit_residual_plot":"SASDCQ6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCQ6_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/CL-9t1.log","software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCQ6_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCQ6_fit2_model1.pdb","model_mw":88.22,"bead_radius":3.5,"log":null,"symmetry":"P1","comment":"SAXNS_DAMMIF","user":85}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCQ6_fit2_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.01,"p_value":0.7139,"fit_residual_plot":"SASDCQ6_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCQ6_fit2.fir","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) in 1mM EDTA, 10mM DTT, 500mM NaCl, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 0.5 mg/ml was measured at 10°C. SAXS data were acquired from 12 successive 21600 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":100.0,"experimental_mw_error":null,"guinier_i0_mw":73.0,"guinier_i0_mw_error":null,"porod_mw":113.0,"porod_mw_error":null,"pddf_i0":0.2183,"pddf_i0_error":0.0012,"guinier_i0":0.2168,"guinier_i0_error":0.002,"pddf_rg":3.41,"pddf_rg_error":0.02,"guinier_rg":3.35,"guinier_rg_error":5.0,"pddf_dmax":11.0,"pddf_dmax_error":null,"porod_volume":180.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":19,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCQ6_datloglog_img.png","symmetry":null,"last_modified":"2020-01-08T13:44:04.490163+01:00","bragg_peak":[]}