{"code":"SASDCQ8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions","publication":null,"status":"released","submitted_date":"2017-11-02","released_date":"2021-09-20"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCQ8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCQ8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bovine serum albumin","short_name":"BSA","sequence":"DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASL\r\nRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKY\r\nNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKV\r\nHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQE\r\nAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEY\r\nGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESL\r\nVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAA\r\nDDKEACFAVEGPKLVVSTQTALA","organism":"Bos taurus","uniprot_code":"P02769","uniprot_range_first":25,"uniprot_range_last":607,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPF\nDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEP\nERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYY\nANKYNGVFQECCQAEDKGACLLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVA\nRLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKE\nCCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRR\nHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEK\nLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLIL\nNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLP\nDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVV\nSTQTALA","mw":66.462,"total_mw":66.462,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"TRIS 50mM","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Bovine serum albumin (BSA)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Clement","contributor_surname":"Blanchet","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-12-21","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.001,"number_of_frames":1,"wavelength":0.124,"sample_detector_distance":1.6,"concentration_min":null,"concentration_max":2.82,"sample_volume":null,"flow_rate":null,"s_min":0.087,"s_max":5.504,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCQ8_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"3v03","ext_code":"3V03","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCQ8_fit1_model1.pdb","model_mw":62.38,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":43}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCQ8_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.055,"p_value":0.405342,"fit_residual_plot":"SASDCQ8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCQ8_fit1.fit","fit_log":null,"software_version":"2.8.3","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCQ8_fit2_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCQ8_fit2_model1.pdb","model_mw":61.1,"bead_radius":2.5,"log":null,"symmetry":"","comment":"","user":43}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCQ8_fit2_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.022,"p_value":4e-06,"fit_residual_plot":"SASDCQ8_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCQ8_fit2.fir","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of bovine serum albumin (BSA) in 50 mM Tris, pH 7.4 were collected at the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using an Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.82 mg/ml was measured at 20°C. A single 1.3 millisecond frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to the protein concentration.","tags":["benchmark"],"intensity_unit":"arbitrary","experimental_mw":62.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":815763.0,"guinier_i0_error":2772.0,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":2.83,"guinier_rg_error":0.1,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":162,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCQ8_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:55:58.257585+02:00","bragg_peak":[]}