{"code":"SASDCR6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.","publication":{"title":"Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.","author_list":"White MA, Tsalkova T, Mei FC, Cheng X","journal":"Cells","doi":"10.3390/cells9010035","pmid":"31877746","published_date":"2019 Dec 21"},"status":"released","submitted_date":"2017-07-27","released_date":"2019-12-02"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCR6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCR6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCR6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCR6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCR6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCR6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCR6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Sealy Center For Structural Biology, UTMB-G","city":"Galveston, TX","country":"USA","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Rap guanine nucleotide exchange factor 3 (dimer)","short_name":"EPAC1B (dimer)","sequence":"GSPGIRVL\r\nVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSES\r\nLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHS\r\nRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVGTHEMEEELA\r\nEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLL\r\nFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA\r\nATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTP\r\nGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHH\r\nFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDT\r\nRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVP\r\nYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVA\r\nTSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSI\r\nHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKL\r\nAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYR\r\nLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPV\r\nPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSREL\r\nEP","organism":"Homo sapiens","uniprot_code":"O95398","uniprot_range_first":2,"uniprot_range_last":881,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLL\nEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRK\nYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQ\nFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEEL\nLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGL\nVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHG\nKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSD\nFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSML\nHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWL\nPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQV\nLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDL\nVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVAT\nELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKV\nRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFE\nKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWA\nYVQQLKVIDNQRELSRLSRELEP","mw":100.001,"total_mw":200.002,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, and 10mM Tris","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":9.0,"deuteration":null},"purity_method":null,"name":"Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Texas, USA","full_name":"University of Texas Medical Branch","webpage":"http://www.bmb.utmb.edu/"}],"contributor_name":"Mark","contributor_surname":"White","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2012-01-30","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":1800.0,"number_of_frames":4,"wavelength":0.15418,"sample_detector_distance":0.489,"concentration_min":null,"concentration_max":4.0,"sample_volume":null,"flow_rate":null,"s_min":0.137,"s_max":6.804,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCR6_fit1_model1_img.png","software":"CORAL","pdb_link":[{"pdb_code":"4zf7","ext_code":"4ZF7","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCR6_fit1_model1.pdb","model_mw":200.9,"bead_radius":1.9,"log":null,"symmetry":"P2","comment":"4xf7 combined with SwissModel 3CF6","user":85}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCR6_fit1_fixed_fit_img.png","software":"CORAL","chi_square_value":1.01,"p_value":0.3291,"fit_residual_plot":"SASDCR6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCR6_fit1.fit","fit_log":null,"software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCR6_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCR6_fit2_model1.pdb","model_mw":213.1,"bead_radius":12.0,"log":null,"symmetry":"P2","comment":"SAXNS_DAMMIF","user":85}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCR6_fit2_fixed_fit_img.png","software":"DAMMIF","chi_square_value":0.958,"p_value":0.5035,"fit_residual_plot":"SASDCR6_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCR6_fit2.fir","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP - in 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 4 mg/ml was measured at 10°C. SAXS data were acquired from 4 successive 1800 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":178.0,"experimental_mw_error":null,"guinier_i0_mw":178.0,"guinier_i0_mw_error":null,"porod_mw":260.0,"porod_mw_error":null,"pddf_i0":1.868,"pddf_i0_error":0.01664,"guinier_i0":1.973,"guinier_i0_error":0.032,"pddf_rg":5.15,"pddf_rg_error":0.03,"guinier_rg":5.32,"guinier_rg_error":0.07,"pddf_dmax":15.7,"pddf_dmax_error":null,"porod_volume":415.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":12,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCR6_datloglog_img.png","symmetry":null,"last_modified":"2020-01-08T13:44:04.702755+01:00","bragg_peak":[]}