{"code":"SASDCS2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.","publication":{"title":"Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.","author_list":"Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC","journal":"Mol Cell","doi":"10.1016/j.molcel.2018.11.006","pmid":"30554943","published_date":"2018 Dec 13"},"status":"released","submitted_date":"2017-04-12","released_date":"2018-09-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCS2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCS2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCS2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCS2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bromodomain-containing protein 3","short_name":"BRD3","sequence":"SMNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDE","organism":"Homo sapiens","uniprot_code":"Q15059","uniprot_range_first":20,"uniprot_range_last":418,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFY\nQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTD\nDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPA\nTPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKK\nGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQ\nHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLST\nVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPV\nEAPALPAPAAPMVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAAL\nSQAPVNKPKKKKEKKEKEKKKKDKEKEKEKHKVKAEEEKKAKVAPPAKQAQQKKAPAKKA\nNSTTTAGRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQ\nSREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCLQKKQRKPFSASGKKQAAKSKEEL\nAQEKKKELEKRLQDVSGQLSSSKKPARKEKPGSAPSGGPSRLSSSSSSESGSSSSSGSSS\nDSSDSE","mw":44.491,"total_mw":44.491,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Bromodomain-containing protein 3 (BRD3) tandem bromodomains","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Oxford, UK","full_name":"University of Oxford","webpage":"http://www.ox.ac.uk/"}],"contributor_name":"Joseph","contributor_surname":"Newman","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-01-13","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":10.0,"number_of_frames":12,"wavelength":0.1,"sample_detector_distance":3.923,"concentration_min":null,"concentration_max":13.0,"sample_volume":null,"flow_rate":null,"s_min":0.04,"s_max":4.398,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCS2_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCS2_fit1_model1.pdb","model_mw":40.755,"bead_radius":4.0,"log":null,"symmetry":"","comment":"","user":208}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCS2_fit1_fit_img.png","software":"DAMMIN","chi_square_value":1.277,"p_value":0.103,"fit_residual_plot":"SASDCS2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCS2_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/brd3-u001.log","software_version":"","description":null}],"estimated_volume_method":"DAMMIN","pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of BRD3 tandem bromodomains in 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). In-line continuous flow HPLC-SAXS was employed at 10°C with a sample injection concentration of 13 mg/ml onto a Shodex 404 column. 12 successive 10 second frames were collected through the HPLC-elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves underwent single-value decomposition to produce the average-median scattering data displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":44.49,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":36.0,"porod_mw_error":null,"pddf_i0":0.1035,"pddf_i0_error":0.0008,"guinier_i0":0.1,"guinier_i0_error":0.0008,"pddf_rg":6.55,"pddf_rg_error":0.054,"guinier_rg":6.22,"guinier_rg_error":0.92,"pddf_dmax":21.5,"pddf_dmax_error":null,"porod_volume":210.0,"porod_volume_error":null,"estimated_volume":81.0,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":71,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCS2_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}