{"code":"SASDCS8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions","publication":null,"status":"released","submitted_date":"2017-11-02","released_date":"2021-09-20"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCS8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCS8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCS8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Eiger 4M","resolution":75.0},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cytochrome C","short_name":"CytC","sequence":"MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITW\r\nGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE","organism":"Bos taurus","uniprot_code":"P62894","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFSYTDANKNKGITW\nGEETLMEYLENPKKYIPGTKMIFAGIKKKGEREDLIAYLKKATNE","mw":11.703,"total_mw":11.703,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"TRIS 50mM","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Cytochrome C","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Clement","contributor_surname":"Blanchet","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-12-21","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.001,"number_of_frames":1,"wavelength":0.124,"sample_detector_distance":1.6,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.035,"s_max":5.57,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of cytochrome C in 50 mM Tris, pH 7.4 were collected at the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using an Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. A single 1.3 millisecond frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled to the protein concentration.","tags":["benchmark"],"intensity_unit":"arbitrary","experimental_mw":8.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":1198940.0,"guinier_i0_error":2464.0,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":1.34,"guinier_rg_error":0.07,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":138,"guinier_point_last":401,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCS8_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:55:58.257585+02:00","bragg_peak":[]}