{"code":"SASDCT6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome.","publication":{"title":"The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome.","author_list":"Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD","journal":"Nucleic Acids Res","doi":"10.1093/nar/gky206","pmid":"29850894","published_date":"2018 Jun 1"},"status":"released","submitted_date":"2017-08-03","released_date":"2018-03-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCT6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCT6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCT6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCT6.sascif","experiment":{"instrument":{"detector":{"type":"Pilatus","name":"Pilatus 200K","resolution":172.0},"name":"Cornell High Energy Synchrotron Source (CHESS)","city":"Ithaca, NY","country":"USA","beamline_name":"G1","beam_geometry":"","type_of_source":"X-ray 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States","full_name":"Cornell University","webpage":"https://www.cornell.edu/"}],"contributor_name":"Joshua","contributor_surname":"Tokuda","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-10-24","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":10.0,"number_of_frames":40,"wavelength":0.1109,"sample_detector_distance":2.08,"concentration_min":null,"concentration_max":1.1,"sample_volume":null,"flow_rate":null,"s_min":0.16,"s_max":2.6,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCT6_fit1_model1_img.png","software":"Custom in-house","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDCT6_fit1_model1.pdb","model_mw":103.9,"bead_radius":1.9,"log":null,"symmetry":null,"comment":"Generated using 3D-DART and custom scripts written in Pymol.  Percentage represents ensemble weight.","user":170}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCT6_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.67,"p_value":0.0646,"fit_residual_plot":"fit4columns1046_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCT6_fit1.fit","fit_log":null,"software_version":"2.1 r7883","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"Version 5.0 (r4547)","i0_calibration_standard":null,"description":"12N12 nucleosome measured in 60% sucrose and ADP-BeF3.  \r\nDue to contrast matching between the solvent and histone proteins (due to sucrose), the measured signal is dominated by the DNA component. The model was generated using 3D-DART and custom scripts written in Pymol. The percentage-weighting of each model in the ensemble ('XXXperc') is noted in the model key below: \r\nSASDCT6_fit1_model1 = 12N12_ADP_01_100perc","experiment_description":"Synchrotron SAXS data from solutions of 12N12 nucleosome in 60% sucrose with ADP-BeF3 in 10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH 7.8, were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (Ithaca, NY, USA) using a Pilatus Pilatus 200K detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 1.10 mg/ml was measured at 25°C. 40 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to produce the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":105.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.0222,"pddf_i0_error":0.00013,"guinier_i0":0.021622,"guinier_i0_error":0.000176,"pddf_rg":4.937,"pddf_rg_error":0.026,"guinier_rg":4.812,"guinier_rg_error":0.52,"pddf_dmax":14.0,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":12,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCT6_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:28:07.375857+02:00","bragg_peak":[]}