{"code":"SASDCT8","status":"Published","type_of_curve":"Other","angular_unit":"1/nm","project":{"title":"Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.","publication":{"title":"Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.","author_list":"de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V","journal":"Mol Cell","doi":"10.1016/j.molcel.2018.02.022","pmid":"29547724","published_date":"2018 Mar 15"},"status":"released","submitted_date":"2017-11-06","released_date":"2018-03-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCT8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCT8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCT8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCT8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCT8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"hypothetical protein CTHT_0072540","short_name":null,"sequence":"MLCALSGEIPEEPVVSKKTGVLFEKRLILKYLEEHNNIEPGTTEELDPETDLLPIKTSRVVRPRPPNFTSIPSLLKAFQDEWDALVLETYTTREQLARVREELATALYQHDAAVRVIARLTRERDEAREALARLTV","organism":"Chaetomium thermophilum","uniprot_code":"G0SFY0","uniprot_range_first":1,"uniprot_range_last":136,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MLCALSGEIPEEPVVSKKTGVLFEKRLILKYLEEHNNIEPGTTEELDPETDLLPIKTSRV\nVRPRPPNFTSIPSLLKAFQDEWDALVLETYTTREQLARVREELATALYQHDAAVRVIARL\nTRERDEAREALARLTVTGAAPAAQNGEAMAVDSESLSEGLVEHVNEVQQQLMKTRKKRPI\nPQGWATADDVAALQQVAYTDLNVTQASSLDLENECAAVGGLDGKLDIYSVVANKVERTLD\nIGEPVTATEWTGTKVVIGTAKGWVKVYDAGRESATFQTHAGPVTGLAVHPGGRILASVGV\nDKSFVFYDLETGERVARGYADAALTTCAFHPDGNLFAAGTQTGHILVFHTTTLEQAESFP\nLGTPIQALAFSENGFWFAATGKGTSSVTIFDLRKSGAAAAVKELQTGEVLSISWDYTGQY\nLATGGGTGVTVQMYTKATKSWSEPVRLGMPVVGVKWGGEAKRLVVVSREGVVSVLGKKEE","mw":15.552,"total_mw":62.206,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Tales","contributor_surname":"Rocha de Moura","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-07-30","storage_temperature":null,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.1,"concentration_min":1.3,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.028,"s_max":4.45,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCT8_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCT8_fit1_model1.pdb","model_mw":40.025,"bead_radius":2.75,"log":null,"symmetry":"","comment":"","user":227}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCT8_fit1_fit_img.png","software":"DAMMIF","chi_square_value":1.041,"p_value":0.02469,"fit_residual_plot":"SASDCT8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCT8_fit1.fir","fit_log":null,"software_version":"","description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCT8_fit2_model1_img.png","software":"SASREF","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCT8_fit2_model1.pdb","model_mw":54.08,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":227}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCT8_fit2_fit_img.png","software":"SASREF","chi_square_value":1.475,"p_value":0.012654,"fit_residual_plot":"fit4columns966_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCT8_fit2.fit","fit_log":null,"software_version":"","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum in 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH 7.5, were collected on the P12 beam line at the PETRAIII the storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 5 mg/ml were measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":"arbitrary","experimental_mw":55.5,"experimental_mw_error":null,"guinier_i0_mw":90.0,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":2398.0,"pddf_i0_error":14.9,"guinier_i0":2368.95,"guinier_i0_error":15.4,"pddf_rg":4.313,"pddf_rg_error":0.045,"guinier_rg":4.09,"guinier_rg_error":0.04,"pddf_dmax":16.2,"pddf_dmax_error":1.5,"porod_volume":145.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":31,"guinier_point_last":108,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCT8_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}