{"code":"SASDCV3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.","publication":{"title":"Biophysical investigation of type A PutAs reveals a conserved core oligomeric structure.","author_list":"Korasick DA, Singh H, Pemberton TA, Luo M, Dhatwalia R, Tanner JJ","journal":"FEBS J","doi":"10.1111/febs.14165","pmid":"28710792","published_date":"2017 Sep"},"status":"released","submitted_date":"2017-05-19","released_date":"2017-07-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCV3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCV3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCV3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCV3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCV3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCV3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCV3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bifunctional protein PutA","short_name":"LpPutA","sequence":"HHHHHHGKPIPNPLLGLDSTENLYFQG\r\nMLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMMAIKTSATKLVQSVRSERKKSTGIDSF\r\nLTEYALSSDEGIALMCLAEALLRVPDNATIDNLIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLT\r\nPEKAENTLTKALLKLVNRSSEAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEA\r\nALTSADAARYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLALSRL\r\nAKDYGIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYVLDWVAALARSKQRRIM\r\nVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAKKILTMTDAIYPQFATHNAYSVAMILNLV\r\nGGYRDFEFQCLHGMGNELYEQIVPANCYGIPCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKA\r\nPISELVEDPVAKSRSLLDKINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMI\r\nAGRKLSRDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFELWSKKPVEERASCLNRFADLLQAN\r\nMAELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFNGYTGELNELSLHPRGTILCISP\r\nWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKLMHQAGIPEGVIQLIPGAGETIGAALVADKR\r\nIKAVLFTGSTDTANLINRTLATRGGEIIPLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSAL\r\nRVLYVQEEVYPRTVELLKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDE\r\nALSGYFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTSYGLTLGIHSRINETVDYIRQ\r\nRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTYTVDTTAAGGNASLMSIPEEG","organism":"Legionella pneumophila subsp. pneumophila","uniprot_code":"Q5ZUU6","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MENIMLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMMAIKTSATKLVQS\nVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDNLIKDKLAGGDWGAH\nRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSSEAVVRKAVDKAMRIMS\nKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAARYFEAYKEAIISIGEKAD\nKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLALSRLAKDYGIALTIDAEESE\nRLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYVLDWVAALARSKQRRIMVRLIKG\nAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAKKILTMTDAIYPQFATHNAYSVAMI\nLNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGIPCRIYAPVGSHEDLLPYLVRRLLENG\nANSSFVNRIVDDKAPISELVEDPVAKSRSLLDKINKNIPLPEDIFLPVRKNSKGFDFTNR\nLERALLQQELAKIESKEWQASPMIAGRKLSRDLLQTVMSPQQPAYAIGSVQQATLDDVEV\nALNQAKLAFELWSKKPVEERASCLNRFADLLQANMAELMVLTCREAGKTWSDGIAEVREA\nIDFCRYYAKKAQELMSSPQRFNGYTGELNELSLHPRGTILCISPWNFPLAIFTGQVVAGL\nVTGNCVIAKPAEQTPLIAAYAVKLMHQAGIPEGVIQLIPGAGETIGAALVADKRIKAVLF\nTGSTDTANLINRTLATRGGEIIPLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQR\nCSALRVLYVQEEVYPRTVELLKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMR\nKHHEILYQCTVDDEALSGYFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQI\nNQTSYGLTLGIHSRINETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAG\nGPNYLIRLCHERTYTVDTTAAGGNASLMSIPEEG","mw":119.178,"total_mw":238.356,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5.","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Proline utilization A from Legionella pneumophila 5 mg/mL","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Mizzou","address":"Columbia, MO, USA","full_name":"University of Missouri-Columbia","webpage":"http://missouri.edu/"}],"contributor_name":"John","contributor_surname":"Tanner","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2010-04-20","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.106,"s_max":3.28,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Proline utilization A from Legionella pneumophila in 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, and 0.5 mM THP at pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged. The  radially averaged scattering of the solvent-blank was subtracted.  X-ray wavelength, unknown; Exposure time, unknown; Sample-To-Detector distance, unknown.","tags":[],"intensity_unit":null,"experimental_mw":240.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1015.0,"pddf_i0_error":0.9,"guinier_i0":1010.6,"guinier_i0_error":1.03,"pddf_rg":4.67,"pddf_rg_error":0.05,"guinier_rg":4.59,"guinier_rg_error":0.01,"pddf_dmax":15.3,"pddf_dmax_error":null,"porod_volume":297.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":29,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCV3_datloglog_img.png","symmetry":null,"last_modified":"2026-05-18T10:07:05.315729+02:00","bragg_peak":[]}