{"code":"SASDCX9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.","publication":{"title":"Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.","author_list":"Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1716614114","pmid":"29229840","published_date":"2017 Dec 26"},"status":"released","submitted_date":"2017-11-29","released_date":"2017-12-12"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCX9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCX9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCX9_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCX9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCX9.sascif","experiment":{"instrument":{"detector":{"type":"Pilatus","name":"Pilatus 200K","resolution":172.0},"name":"Cornell High Energy Synchrotron Source (CHESS)","city":"Ithaca, NY","country":"USA","beamline_name":"G1","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bacteriophage phi-X174","short_name":null,"sequence":"NA","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":0.203,"total_mw":0.203,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Bacteriophage phi-X174 - ~545s after mixing with LPS","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Ithaca, NY, United States","full_name":"Cornell University","webpage":"https://www.cornell.edu/"}],"contributor_name":"Alex","contributor_surname":"Mauney","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-10-25","storage_temperature":0.0,"cell_temperature":33.0,"exposure_time":10.0,"number_of_frames":10,"wavelength":0.1109,"sample_detector_distance":2.052,"concentration_min":null,"concentration_max":0.1,"sample_volume":null,"flow_rate":null,"s_min":0.06,"s_max":2.6,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"Note: Due to the size of the capsids, the Rg and I(0) obtained from Guinier analysis should be interpreted as approximated estimates only.","experiment_description":"Synchrotron SAXS data from solutions of Bacteriophage phi-X174 ~545 s after mixing with Lipopolysaccharide (LPS) at 0.15 mg/mL in 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH 7.4 were collected on the BioSAXS G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Pilatus Pilatus 200K detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1109 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.10 mg/ml phage was measured at 33°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":0.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":21.3,"guinier_i0_error":0.2,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":12.88,"guinier_rg_error":0.04,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCX9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}