{"code":"SASDCZ7","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.","publication":{"title":"Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.","author_list":"Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R","journal":"Nat Biotechnol","doi":"10.1038/nbt.3994","pmid":"29035374","published_date":"2017 Nov"},"status":"released","submitted_date":"2017-09-28","released_date":"2017-10-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDCZ7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDCZ7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCZ7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCZ7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDCZ7.sascif","experiment":{"instrument":{"detector":{"type":"Custom-built","name":"Pilatus 1M-W","resolution":0.172},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"TET12(1.6)S-c6b","short_name":null,"sequence":"MQLEDKVEELLSKNYHLENEVSRLKKLVGGDGKGSPEDEIQQLEEEISQLEQKNSQLKEKNQQLKYG  GKGDGDIEQELERAKQSIRRLEQEVNQERSRMQYLQTLLSKGKGDGQLEDKVEELLSKNYHLENEVSRLKKLVGGKGDGLEEELKQLEEELQAIEEQLAQLQWKAQARKEKLAQLKEKLGKGDGSPEDENSQLEEKISQLKQKNSQLKEEIQQLEYGGKGDGDIEQELERAKQSIRRLEQEVNQERSRMQYLQTLLSKGKGDGSPEDEIQSLEEKNSQLKQEISQLEEKNQQLKYGGKGDGLEEELKQLEEELQAIEEQLAQLQWKAQARKE KLAQLKEKLGKGDGSPEDKISQLKQKIQQLKQENQQLEEENSQLEYGGDGKGSPEDKISQLKEENQQLEQKIQQLKEENSQLEYGGDGKGSPEDKNSQLKEEIQQLEEENQQLEEKISQLKYGLEHHHHHHHH","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":54.468,"total_mw":54.468,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris 150 mM NaCl 10% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"TET12(1.6)S-c6b","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"National Research Council","webpage":null}],"contributor_name":"Jana","contributor_surname":"Aupič","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2016-04-07","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.045,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":2.91,"sample_volume":null,"flow_rate":null,"s_min":0.182,"s_max":6.716,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDCZ7_fit1_model1_img.png","software":"MODELLER","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"9.16","model_data":"https://www.sasbdb.org/media/pdb_file/SASDCZ7_fit1_model1.pdb","model_mw":54.43,"bead_radius":1.9,"log":null,"symmetry":"","comment":"","user":201}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCZ7_fit1_fit_img.png","software":"Other","chi_square_value":1.01,"p_value":0.0,"fit_residual_plot":"SASDCZ7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDCZ7_fit1.fit","fit_log":null,"software_version":"PepsiSAXS","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of TET12(1.6)S-c6b in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. Scattering curves were merged and analyzed using PRIMUS software.","tags":[],"intensity_unit":null,"experimental_mw":53.2,"experimental_mw_error":null,"guinier_i0_mw":53.2,"guinier_i0_mw_error":0.4,"porod_mw":67.0,"porod_mw_error":null,"pddf_i0":7157.0,"pddf_i0_error":41.0,"guinier_i0":7255.14,"guinier_i0_error":29.0,"pddf_rg":3.55,"pddf_rg_error":0.02,"guinier_rg":3.54,"guinier_rg_error":0.05,"pddf_dmax":11.5,"pddf_dmax_error":1.0,"porod_volume":114.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":33,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDCZ7_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:18.120449+02:00","bragg_peak":[]}