{"code":"SASDD22","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.","publication":{"title":"Cryptic Disorder Out of Disorder: Encounter between Conditionally Disordered CP12 and Glyceraldehyde-3-Phosphate Dehydrogenase.","author_list":"Launay H, Barré P, Puppo C, Zhang Y, Maneville S, Gontero B, Receveur-Bréchot V","journal":"J Mol Biol","doi":"10.1016/j.jmb.2018.02.020","pmid":"29501381","published_date":"2018 Apr 13"},"status":"released","submitted_date":"2017-12-04","released_date":"2018-03-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDD22.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDD22.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD22_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD22_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDD22.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"AVIEX PCCD170170","resolution":null},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":"","type_of_source":"X-ray 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dehydrogenase","short_name":"GAPDH","sequence":"EKKIRVAINGFGRIGRNFLRCWHGRQNTLLDVVAINDSGGVKQASHLLKYDSTLGTFAADVKIVDDSHISVDGKQIKIVSSRDPLQLPWKEMNIDLVIEGTGVFIDKVGAGKHIQAGASKVLITAPAKDKDIPTFVVGVNEGDYKHEYPIISNASCTTNCLAPFVKVLEQKFGIVKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTTTGAAKAVSLVLPSLKGKLNGIALRVPTPTVSVVDLVVQVEKKTFAEEVNAAFREAANGPMKGVLHVEDAPLVSIDFKCTDQSTSIDASLTMVMGDDMVKVVAWYDNEWGYSQRVVDLAEVTAKKWVA","organism":"Chlamydomonas 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chloroplastic calvin cycle protein CP12 (C. reinhardtii)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Marseille, France","full_name":"Aix Marseille Univ, CNRS, BIP UMR7281","webpage":"http://bip.cnrs-mrs.fr"}],"contributor_name":"Veronique","contributor_surname":"Receveur-Brechot","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2010-12-08","storage_temperature":15.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":250,"wavelength":0.1033,"sample_detector_distance":1.832,"concentration_min":null,"concentration_max":16.0,"sample_volume":null,"flow_rate":null,"s_min":0.105,"s_max":4.595,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD22_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":5.291,"p_value":0.0,"fit_residual_plot":"fit4columns1223_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD22_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_8Gj01w3.log","software_version":null,"description":null},{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD22_fit2_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":29.721,"p_value":0.0,"fit_residual_plot":"fit4columns1224_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD22_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/Size_distr_003_1.dat","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Glyceraldehyde-3-phosphate dehydrogenase in complex with oxidised chloroplastic calvin cycle protein CP12 in 30 mM Tris, 4 mM EDTA, 100 µM NAD, 5 mM free cysteine, pH 7.9 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a CCD AVIEX detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 16.00 mg/ml was measured at 20°C. 250 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.","tags":[],"intensity_unit":"1/cm","experimental_mw":166.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.0003877,"pddf_i0_error":2e-07,"guinier_i0":0.0003852,"guinier_i0_error":1e-08,"pddf_rg":3.99,"pddf_rg_error":0.006,"guinier_rg":3.84,"guinier_rg_error":0.01,"pddf_dmax":19.0,"pddf_dmax_error":1.0,"porod_volume":250.82,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":31,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDD22_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:27:27.968395+02:00","bragg_peak":[]}