{"code":"SASDD29","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"NAD<sup>+</sup> Promotes Assembly of the Active Tetramer of Aldehyde Dehydrogenase 7A1.","publication":{"title":"NAD<sup>+</sup> Promotes Assembly of the Active Tetramer of Aldehyde Dehydrogenase 7A1.","author_list":"Korasick DA, White TA, Chakravarthy S, Tanner JJ","journal":"FEBS Lett","doi":"10.1002/1873-3468.13238","pmid":"30184263","published_date":"2018 Sep 5"},"status":"released","submitted_date":"2018-06-22","released_date":"2018-09-10"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDD29.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD29_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD29_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD29_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDD29.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Advanced Photon Source (APS), Argonne National Laboratory","city":"Lemont, IL","country":"USA","beamline_name":"BioCAT 18ID","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Alpha-aminoadipic semialdehyde dehydrogenase","short_name":"ALDH7A1","sequence":">ALDH7A1\r\nGHMSTLLINQPQYAWLKELGLREENEGVYNGSWGGRGEVITTYCPANNEPIARVRQASVADYEETVKKAREAWKIWADIPAPKRGEIVRQIGDALREKIQVLGSLVSLEMGKILVEGVGEVQEYVDICDYAVGLSRMIGGPILPSERSGHALIEQWNPVGLVGIITAFNFPVAVYGWNNAIAMICGNVCLWKGAPTTSLISVAVTKIIAKVLEDNKLPGAICSLTCGGADIGTAMAKDERVNLLSFTGSTQVGKQVGLMVQERFGRSLLELGGNNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEVVNRLKKAYAQIRVGNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHDASIAHTETFAPILYVFKFKNEEEVFAWNNEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGRESGSDAWKQYMRRSTCTINYSKDLPLAQGIKFQ","organism":"Homo sapiens","uniprot_code":"P49419","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MWRLPRALCVHAAKTSKLSGPWSRPAAFMSTLLINQPQYAWLKELGLREENEGVYNGSWG\nGRGEVITTYCPANNEPIARVRQASVADYEETVKKAREAWKIWADIPAPKRGEIVRQIGDA\nLREKIQVLGSLVSLEMGKILVEGVGEVQEYVDICDYAVGLSRMIGGPILPSERSGHALIE\nQWNPVGLVGIITAFNFPVAVYGWNNAIAMICGNVCLWKGAPTTSLISVAVTKIIAKVLED\nNKLPGAICSLTCGGADIGTAMAKDERVNLLSFTGSTQVGKQVGLMVQERFGRSLLELGGN\nNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEVVNRLKKAYAQIRVG\nNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHD\nASIAHTETFAPILYVFKFKNEEEVFAWNNEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGI\nVNVNIPTSGAEIGGAFGGEKHTGGGRESGSDAWKQYMRRSTCTINYSKDLPLAQGIKFQ","mw":55.56,"total_mw":222.24,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris, 50 mM NaCl, 0.5 mM DTT, 5% (v/v) glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.8,"deuteration":null},"purity_method":null,"name":"Low load concentration of apo alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1) collected by SEC-SAXS","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Mizzou","address":"Columbia, MO, USA","full_name":"University of Missouri-Columbia","webpage":"http://missouri.edu/"}],"contributor_name":"David","contributor_surname":"Korasick","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-22","storage_temperature":null,"cell_temperature":22.0,"exposure_time":0.5,"number_of_frames":null,"wavelength":0.103,"sample_detector_distance":3.5,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.081,"s_max":3.87,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD29_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"4zuk","ext_code":"4ZUK","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDD29_fit1_model1.pdb","model_mw":216.0,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":290}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD29_fit1_fitfoxs_img.png","software":"FoXS","chi_square_value":1.153286,"p_value":0.0,"fit_residual_plot":"SASDD29_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD29_fit1.dat","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD29_fit2_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"4zuk","ext_code":"4ZUK","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDD29_fit2_model1.pdb","model_mw":108.1,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":290},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD29_fit2_model2_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"4zuk","ext_code":"4ZUK","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDD29_fit2_model2.pdb","model_mw":216.0,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":290}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD29_fit2_fitfoxs_img.png","software":"MultiFoXS","chi_square_value":1.030374,"p_value":0.0,"fit_residual_plot":"SASDD29_fit2_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD29_fit2.dat","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"Column type: Wyatt WTC 030 S5; flow rate: 0.8 mL/min; injection volume: 250 - 500 µL","experiment_description":"Synchrotron SAXS data from solutions of apo alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1) in 50 mM Tris, 50 mM NaCl, 0.5 mM DTT, 5% (v/v) glycerol, pH 7.8 were collected using SEC-SAXS on the BioCAT 18ID beam line at the Advanced Photon Source (APS; Argonne, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were recorded from samples at 22°C.","tags":[],"intensity_unit":"arbitrary","experimental_mw":100.4,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":42.4,"guinier_i0_error":0.4,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":3.47,"guinier_rg_error":0.05,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":212.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":98,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDD29_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}