{"code":"SASDD43","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"MbsTA","publication":null,"status":"released","submitted_date":"2018-01-16","released_date":"2019-12-02"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDD43.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDD43.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD43_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD43_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD43_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD43_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDD43.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Mycobacterial cidal toxin","short_name":"MbcT","sequence":">MbsT\r\nMDSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTTAEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQRTRPGQLQLRQSVDLTPALYQELRAT","organism":"Mycobacterium tuberculosis H37Rv","uniprot_code":"P9WLP9","uniprot_range_first":1,"uniprot_range_last":186,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLA\nDSAQACMVEVERAAQAASTTAEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDI\nYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQRTRPGQLQLRQSVDLTPALY\nQELRAT","mw":20.396,"total_mw":20.396,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"30 mM Tris-HCl, 200 mM NaCl, 10% glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Mycobacterial suicide toxin (MbsT)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Diana","contributor_surname":"Freire","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-05-02","storage_temperature":20.0,"cell_temperature":20.3,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":2.0,"concentration_min":0.4,"concentration_max":7.4,"sample_volume":null,"flow_rate":null,"s_min":0.108,"s_max":4.835,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD43_fit1_model1_img.png","software":"SREFLEX","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDD43_fit1_model1.pdb","model_mw":19.94,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/log.txt","symmetry":null,"comment":null,"user":277}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD43_fit1_fit_img.png","software":"SREFLEX","chi_square_value":1.221,"p_value":0.569452,"fit_residual_plot":"SASDD43_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD43_fit1.fit","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD43_fit2_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6fkg","ext_code":"6FKG","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDD43_fit2_model1.pdb","model_mw":20.21,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":277}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD43_fit2_fit_img.png","software":"CRYSOL","chi_square_value":2.204,"p_value":0.0,"fit_residual_plot":"SASDD43_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD43_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/MbsT_crysol.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Mycobacterial suicide toxin (MbsT) in 30 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH 7.5, were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 7.4 mg/ml were measured at 20.3°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":18.0,"experimental_mw_error":null,"guinier_i0_mw":18.0,"guinier_i0_mw_error":2.0,"porod_mw":17.0,"porod_mw_error":null,"pddf_i0":18610.0,"pddf_i0_error":14.0,"guinier_i0":18612.1,"guinier_i0_error":21.0,"pddf_rg":1.747,"pddf_rg_error":0.002,"guinier_rg":1.75,"guinier_rg_error":0.1,"pddf_dmax":5.6,"pddf_dmax_error":null,"porod_volume":30.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":231,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDD43_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}