{"code":"SASDD86","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease.","publication":{"title":"Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease.","author_list":"Biterova E, Ignatyev A, Uusimaa J, Hinttala R, Ruddock LW","journal":"PLoS One","doi":"10.1371/journal.pone.0202391","pmid":"30138417","published_date":"2018"},"status":"released","submitted_date":"2018-04-18","released_date":"2018-08-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDD86.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDD86.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD86_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD86_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD86_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD86_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDD86.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"NHL repeat-containing protein 2","short_name":"NHLRC2","sequence":"MHHHHHHMAAPGGRGRSLSGLLPAQTSLEYALLDAVTQQEKDSLVYQYLQKVDGWEQDLSVPEFPEGLEWLNTEEPISVYKDLCGKIVVLDFFTYCCINCIHLLPDLHALEHTYSDKDGLLIIGVHSAKFPNEKVLDNIKSAVLRYNITHPMVNDADASLWQELEVSCWPTLVILGPRGNMLFSLIGEGHKDKLFLYTSIALKYYKDRGQIRDNKIGIKLYKDSLPPSPLLFPGKVTVDQVTDRLVIADTGHHRILVVWKNGQIQYSIGGPNPGRKDGIFSESTFNSPQGVAIMNNIIYVADTENHLIRKIDLEAEKVSTVAGIGIQGTDKEGGAKGEQQPISSPWDVVFGTSGSEVQRGDILWIAMAGTHQIWALLLDSGKLPKKNELTKGTCLRFAGSGNEENRNNAYPHKAGFAQPSGLSLASEDPWSCLFVADSESSTVRTVSLKDGAVKHLVGGERDPMNLFAFGDVDGVGINAKLQHPLGVTWDKKRNLLYVADSYNHKIKVVDPKTKNCTTLAGTGDTNNVTSSSFTESTFNEPGGLCIGENGELLYVADTNNHQIKVMDLETKMVSVLPIFRSENAVVDGPFLVEKQKTLPKLPKSAPSIRLSPVTACAGQTLQFKLRLDLPSGSKLTEGVSSCWFLTAEGNEWLLQGQIAAGDIENISSQPTISLQIPDDCLSLEAIVSVSVFLYYCSADSSACMMKAILFSQPLQITDTQQGCIAPVELRYVF","organism":"Homo sapiens","uniprot_code":"Q8NBF2","uniprot_range_first":1,"uniprot_range_last":726,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MAAPGGRGRSLSGLLPAQTSLEYALLDAVTQQEKDSLVYQYLQKVDGWEQDLSVPEFPEG\nLEWLNTEEPISVYKDLCGKIVVLDFFTYCCINCIHLLPDLHALEHTYSDKDGLLIIGVHS\nAKFPNEKVLDNIKSAVLRYNITHPMVNDADASLWQELEVSCWPTLVILGPRGNMLFSLIG\nEGHKDKLFLYTSIALKYYKDRGQIRDNKIGIKLYKDSLPPSPLLFPGKVTVDQVTDRLVI\nADTGHHRILVVWKNGQIQYSIGGPNPGRKDGIFSESTFNSPQGVAIMNNIIYVADTENHL\nIRKIDLEAEKVSTVAGIGIQGTDKEGGAKGEQQPISSPWDVVFGTSGSEVQRGDILWIAM\nAGTHQIWALLLDSGKLPKKNELTKGTCLRFAGSGNEENRNNAYPHKAGFAQPSGLSLASE\nDPWSCLFVADSESSTVRTVSLKDGAVKHLVGGERDPMNLFAFGDVDGVGINAKLQHPLGV\nTWDKKRNLLYVADSYNHKIKVVDPKTKNCTTLAGTGDTNNVTSSSFTESTFNEPGGLCIG\nENGELLYVADTNNHQIKVMDLETKMVSVLPIFRSENAVVDGPFLVEKQKTLPKLPKSAPS\nIRLSPVTACAGQTLQFKLRLDLPSGSKLTEGVSSCWFLTAEGNEWLLQGQIAAGDIENIS\nSQPTISLQIPDDCLSLEAIVSVSVFLYYCSADSSACMMKAILFSQPLQITDTQQGCIAPV\nELRYVF","mw":80.4,"total_mw":80.4,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM BisTris, 100 mM NaCl, 2 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Full-length human NHLRC2 ( NHL repeat-containing protein 2 )","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Finland","full_name":"University of Oulu","webpage":"https://www.oulu.fi"}],"contributor_name":"Alexander","contributor_surname":"Ignatyev","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-07-08","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":3.0,"number_of_frames":60,"wavelength":0.1,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.039,"s_max":3.9,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD86_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"DAMFILT5.0","model_data":"https://www.sasbdb.org/media/pdb_file/SASDD86_fit1_model1.pdb","model_mw":77.76,"bead_radius":3.75,"log":null,"symmetry":"P1","comment":"12 models were averaged and filtered","user":311}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD86_fit1_fit_img.png","software":"DAMMIF","chi_square_value":2.682,"p_value":0.10889,"fit_residual_plot":"SASDD86_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD86_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/NHLRC2_4.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of full-length human NHLRC2 (NHL repeat-containing protein 2 ) in 20 mM BisTris, 100 mM NaCl, 2 mM DTT, pH 6.5, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 60 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":71.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":71.2,"porod_mw_error":null,"pddf_i0":0.3031,"pddf_i0_error":0.0011,"guinier_i0":0.3008,"guinier_i0_error":0.0004,"pddf_rg":3.76,"pddf_rg_error":0.027,"guinier_rg":3.59,"guinier_rg_error":0.1,"pddf_dmax":14.69,"pddf_dmax_error":null,"porod_volume":121.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":14,"guinier_point_last":143,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDD86_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}