{"code":"SASDD95","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.","publication":{"title":"Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.","author_list":"Liu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G","journal":"J Mol Biol","doi":"10.1016/j.jmb.2018.08.026","pmid":"30193986","published_date":"2018 Oct 19"},"status":"released","submitted_date":"2018-03-22","released_date":"2018-09-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDD95.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDD95.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD95_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD95_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDD95_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDD95_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDD95.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Sealy Center For Structural Biology, UTMB-G","city":"Galveston, TX","country":"USA","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Neurexin 1a L5L6","short_name":"L5L6","sequence":"ADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLH\r\nYVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLK\r\nSDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERG\r\nCEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRP\r\nSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNA\r\nIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQGQPFQG\r\nQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVG\r\nEVPSASTSHHHHHH","organism":null,"uniprot_code":"Q9ULB1","uniprot_range_first":904,"uniprot_range_last":1307,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGTALLQRGGCFLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPKWNACCESEMSFQLKT\nRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLLADTPVNDGAWHSVRIR\nRQFRNTTLFIDQVEAKWVEVKSKRRDMTVFSGLFVGGLPPELRAAALKLTLASVREREPF\nKGWIRDVRVNSSQVLPVDSGEVKLDDEPPNSGGGSPCEAGEEGEGGVCLNGGVCSVVDDQ\nAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKEEYIATFKGSEYFCYDLSQNP\nIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNG\nKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSP\nSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQGDPKMKIHGVVAFKCENVATLD\nPITFETPESFISLPKWNAKKTGSISFDFRTTEPNGLILFSHGKPRHQKDAKHPQMIKVDF\nFAIEMLDGHLYLLLDMGSGTIKIKALLKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTA\nPGESEILDLDDELYLGGLPENKAGLVFPTEVWTALLNYGYVGCIRDLFIDGQSKDIRQMA\nEVQSTAGVKPSCSKETAKPCLSNPCKNNGMCRDGWNRYVCDCSGTGYLGRSCEREATVLS\nYDGSMFMKIQLPVVMHTEAEDVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTV\nNLDCIRINCNSSKGPETLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHT\nRLEFHNIETGIITERRYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFR\nNIIADPVTFKTKSSYVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKG\nYLHYVFDLGNGANLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNL\nDLKSDLYIGGVAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGC\nEGPSTTCQEDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWP\nPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAI\nEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTIFNSQATIIIGGKEQG\nQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQSEMSTS\nIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEPSSGGLANPTR\nAGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRN\nYISNSAQSNGAVVKEKQPSSAKSSNKNKKNKDKEYYV","mw":44.499,"total_mw":44.499,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2","concentration_unit":null,"comment":"N1a Buffer","additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Neurexin 1a L5L6","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Texas, USA","full_name":"University of Texas Medical Branch","webpage":"http://www.bmb.utmb.edu/"}],"contributor_name":"Mark","contributor_surname":"White","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-09-06","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":10.0,"number_of_frames":3,"wavelength":0.15418,"sample_detector_distance":0.4838,"concentration_min":2.0,"concentration_max":4.0,"sample_volume":null,"flow_rate":null,"s_min":0.165,"s_max":6.854,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDD95_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":"05","model_data":"https://www.sasbdb.org/media/pdb_file/SASDD95_fit1_model1.pdb","model_mw":44.84,"bead_radius":1.9,"log":null,"symmetry":"P1","comment":null,"user":85}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD95_fit1_fixed_fit_img.png","software":"CORAL","chi_square_value":1.3,"p_value":0.000154,"fit_residual_plot":"SASDD95_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD95_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/cL5a6m.log","software_version":"05","description":null},{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDD95_fit2_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":4.801,"p_value":0.0,"fit_residual_plot":"SASDD95_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDD95_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_011_1.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Neurexin 1a L5L6 in 20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2, pH 8 were collected on a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) using a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 4 mg/ml were measured at 10°C. Three successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":39.8,"experimental_mw_error":null,"guinier_i0_mw":39.6,"guinier_i0_mw_error":0.1,"porod_mw":43.0,"porod_mw_error":null,"pddf_i0":39110.0,"pddf_i0_error":89.0,"guinier_i0":39647.6,"guinier_i0_error":48.5,"pddf_rg":3.0,"pddf_rg_error":0.01,"guinier_rg":3.02,"guinier_rg_error":0.01,"pddf_dmax":10.0,"pddf_dmax_error":0.5,"porod_volume":69.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":17,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDD95_datloglog_img.png","symmetry":null,"last_modified":"2021-08-18T17:28:22.895829+02:00","bragg_peak":[]}