{"code":"SASDDA6","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.","publication":{"title":"A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.","author_list":"Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A","journal":"FEBS J","doi":"10.1111/febs.14993","pmid":"31291689","published_date":"2019 Jul 10"},"status":"released","submitted_date":"2018-05-28","released_date":"2019-07-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDA6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDA6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDA6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDA6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDA6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Rayonix MX225-HE","resolution":null},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Chitinase 2","short_name":"ChiAt2","sequence":">ChiAt2 A.tequilana\r\nSNAQQCGSQAGGAVCPNGLCCSQFGYCGSTSPYCGNGCQSQCGGGSSPTPNPPSGGGGGG\r\nGGGGSGVGSIISSSLFDQMLLHRNDAACPANGFYTYDAFVAAANAFSGFATTGDADTQKR\r\nEIAAFLAQTSHETTGGWPTAPDGPYSWGYCFLQEQGNPGDYCVPNDQWPCAPGKKYYGRG\r\nPIQISYNYNYGPCGNAIRSDLLNNPDLVASDPTVSFKTALWFWMTPQSPKPSCHDVITRA\r\nWTPSAADQAAGRVPGFGVITNIINGGVECGHGSDSRDEDRVGFYKRYCDILGVSFGDNLD\r\nCGNQSHF","organism":"Agave tequilana","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":31.9,"total_mw":31.9,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"MES 50 mM","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.0,"deuteration":null},"purity_method":null,"name":"Class I chitinase 2 from Agave tequilana","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Mexico","full_name":"IQ, UNAM","webpage":"https://www.iquimica.unam.mx/"}],"contributor_name":"Yusvel","contributor_surname":"Sierra-Gómez","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-04-19","storage_temperature":4.0,"cell_temperature":15.0,"exposure_time":1.0,"number_of_frames":null,"wavelength":0.1127,"sample_detector_distance":1.8,"concentration_min":0.46,"concentration_max":7.5,"sample_volume":null,"flow_rate":null,"s_min":0.067,"s_max":3.226,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDA6_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDA6_fit1_model1.pdb","model_mw":24.942,"bead_radius":2.5,"log":"https://www.sasbdb.org/media/log_files/merge_scatter_cut-1.log","symmetry":null,"comment":null,"user":310}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDA6_fit1_fit_img.png","software":"DAMMIF","chi_square_value":2.244,"p_value":0.001073,"fit_residual_plot":"SASDDA6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDA6_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/merge_scatter_cut-1.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Chitinase 2 from Agave tequilana in MES 50 mM, pH 6, were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 7.5 mg/ml were measured at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":31.9,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":108.4,"pddf_i0_error":null,"guinier_i0":107.28,"guinier_i0_error":0.13,"pddf_rg":2.51,"pddf_rg_error":null,"guinier_rg":2.38,"guinier_rg_error":0.047,"pddf_dmax":9.75,"pddf_dmax_error":null,"porod_volume":49.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":83,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDA6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}