{"code":"SASDDB7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Synthetic FRET biosensor Twitch 2B","publication":null,"status":"released","submitted_date":"2018-05-25","released_date":"2021-12-20"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDB7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB7_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDB7.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Synthetic FRET biosensor Twitch2B N532F","short_name":"TW6","sequence":"MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT\r\nTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF\r\nFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGN\r\nVYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNH\r\nYLSTQSKLSKDPNEKRDHMVLLEFVTAARMQVADASEEELSECFRIFDFD\r\nGNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMV\r\nENVQPIYPELMGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPN\r\nEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVE\r\nLDGDVNGHKFSVRGEGEGDATNGKLTLKLICTTGKLPVPWPTLVTTLGYG\r\nLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD\r\nTLVNRIELKGIDFKEDGNILGHKLEYNYNSHFVYITADKQKNGIKANFKI\r\nRHNIED","organism":"synthetic construct","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":62.432,"total_mw":62.432,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM MOPS 100 mM NaCl 5 mM CaCl2","concentration_unit":null,"comment":"Buffer A","additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Synthetic FRET biosensor Twitch 2B N532F Ca-bound","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"Max Planck Institute for Biophysical Chemistry","webpage":"https://www.mpibpc.mpg.de"}],"contributor_name":"Pablo","contributor_surname":"Trigo Mourino","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-06-30","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":12,"wavelength":0.0995,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.035,"s_max":4.952,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the synthetic FRET biosensor, Twitch 2B N532F, bound to calcium, in 20 mM MOPS 100 mM NaCl 5 mM CaCl2, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0995 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 12 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":56.05,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":37.44,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":2.76,"guinier_rg_error":null,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":62,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDB7_datloglog_img.png","symmetry":null,"last_modified":"2021-12-20T11:10:57.905086+01:00","bragg_peak":[]}