{"code":"SASDDB9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.","publication":{"title":"The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.","author_list":"Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B","journal":"J Struct Biol","doi":"10.1016/j.jsb.2018.08.008","pmid":"30110657","published_date":"2018 Aug 12"},"status":"released","submitted_date":"2018-06-29","released_date":"2018-08-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDB9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDB9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDB9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDB9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDB9.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"VNG0258H/RosR","short_name":"VNG0258H/RosR","sequence":">VNG0258H/RosR\r\nMSEAQPDARSDARDLTAFQKNILTVLGEEARYGLAIKRELEEYYGEEVNHGRLYPNLDDL\r\nVNKGLVEKSELDKRTNEYALTNEGFDAVVDDLEWTLSKFVADADRRERVETIVADDAAAL\r\nEHHHHHH","organism":"Halobacterium salinarum NRC-1","uniprot_code":"Q9HSF4","uniprot_range_first":1,"uniprot_range_last":116,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSEAQPDARSDARDLTAFQKNILTVLGEEARYGLAIKRELEEYYGEEVNHGRLYPNLDDL\nVNKGLVEKSELDKRTNEYALTNEGFDAVVDDLEWTLSKFVADADRRERVETIVADD","mw":14.533,"total_mw":29.066,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM HEPES, 2 M NaBr, 0.02% NaN3","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaBr","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Israel","full_name":"Ben-Gurion University of the Negev","webpage":"https://in.bgu.ac.il/"}],"contributor_name":"Nitzan","contributor_surname":"kutnowski","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-26","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.09919,"sample_detector_distance":2.869,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.036,"s_max":4.912,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDB9_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDB9_fit1_model1.pdb","model_mw":10.975,"bead_radius":2.9,"log":null,"symmetry":null,"comment":null,"user":339},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/2M_NaBr_SASDDB9_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5","model_data":"https://www.sasbdb.org/media/pdb_file/2M_NaBr_SASDDB9.pdb","model_mw":11.819,"bead_radius":2.0,"log":null,"symmetry":null,"comment":null,"user":339}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDB9_fit1_fit_img.png","software":"DAMMIN","chi_square_value":1.32555,"p_value":0.002142,"fit_residual_plot":"SASDDB9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDB9_fit1.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS 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One solute concentration of 3.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":null,"experimental_mw":33.4,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":37.0,"porod_mw_error":null,"pddf_i0":17.34,"pddf_i0_error":null,"guinier_i0":18.04,"guinier_i0_error":0.066,"pddf_rg":2.43,"pddf_rg_error":null,"guinier_rg":2.54,"guinier_rg_error":0.31,"pddf_dmax":8.1,"pddf_dmax_error":null,"porod_volume":59.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":42,"guinier_point_last":100,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDB9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}