{"code":"SASDDH2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"C<sub>H</sub>2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.","publication":{"title":"C<sub>H</sub>2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.","author_list":"Yageta S, Imamura H, Shibuya R, Honda S","journal":"MAbs","doi":"10.1080/19420862.2018.1546086","pmid":"30513259","published_date":"2018 Dec 4"},"status":"released","submitted_date":"2018-12-08","released_date":"2018-12-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDH2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDH2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDH2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDH2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"Photon Factory (PF), High Energy Accelerator Research Organization (KEK)","city":"Tsukuba","country":"Japan","beamline_name":"BL-10C","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Aglycosylated human immunoglobulin G Fc region","short_name":"aFc","sequence":"ADKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","organism":"Homo sapiens","uniprot_code":"P01857","uniprot_range_first":104,"uniprot_range_last":330,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS\nGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG\nPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYN\nSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDE\nLTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW\nQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","mw":25.651,"total_mw":51.302,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Citrate-Phosphate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Aglycosylated Human Immunoglobulin G Fc Region","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"AIST","address":"Tokyo, Japan","full_name":"National Institute of Advanced Industrial Science and Technology (AIST)","webpage":"http://www.aist.go.jp"}],"contributor_name":"Seiki","contributor_surname":"Yageta","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-03-05","storage_temperature":null,"cell_temperature":25.0,"exposure_time":2.0,"number_of_frames":15,"wavelength":0.12,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":3.19,"sample_volume":null,"flow_rate":null,"s_min":0.056,"s_max":3.497,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDH2_fit1_model1_img.png","software":"MODELLER","pdb_link":[{"pdb_code":"1hzh","ext_code":"1HZH","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDH2_fit1_model1.pdb","model_mw":51.24,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":270}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDH2_fit1_fit_img.png","software":"CRYSOL","chi_square_value":0.026,"p_value":0.010176,"fit_residual_plot":"SASDDH2_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDH2_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of aglycosylated Human Immunoglobulin G (Fc Region) in 20 mM Citrate-Phosphate, pH 7 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.19 mg/ml was measured at 25°C. 15 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":51.297,"experimental_mw_error":null,"guinier_i0_mw":50.6,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1849.0,"pddf_i0_error":null,"guinier_i0":0.036,"guinier_i0_error":0.0,"pddf_rg":2.85,"pddf_rg_error":null,"guinier_rg":2.87,"guinier_rg_error":0.2,"pddf_dmax":9.8,"pddf_dmax_error":null,"porod_volume":60.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":39,"guinier_point_last":253,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDH2_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}