{"code":"SASDDH6","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.","publication":{"title":"Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.","author_list":"Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P","journal":"Biochemistry","doi":"10.1021/acs.biochem.0c00830","pmid":"33416314","published_date":"2021 Jan 8"},"status":"released","submitted_date":"2018-05-10","released_date":"2020-03-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDH6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDH6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDH6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDH6.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"Rayonix SX165","resolution":15.0},"name":"Synchrotron Light Research Institute (SLRI)","city":"Nakhon Ratchasima","country":"Thailand","beamline_name":"BL1.3W","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis","short_name":"Di-domain ACP","sequence":">PigHACP1ACP2\r\nGSHMNDVTTETYETLKQSVLHTFAQLTGYNVSELSLTSHLENDLGVDSIALAEIAVSLSRQFQLNTPLLIQDINTIKDALDGILQREFQLSEKVEPAAIALSGDADLWLGNLVRQIFASHSGYDVNALALDAEIESDLGIDSVSVASAQGELFNTLQLNSETIIANCNTLSALKQCLAARLVQEKGQDWFELTRAPPPPPLRSGC","organism":"Serratia sp. ATCC 39006","uniprot_code":"Q5W264","uniprot_range_first":4,"uniprot_range_last":191,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNDVTTETYETLKQSVLHTFAQLTGYNVSELSLTSHLENDLGVDSIALAEIAVSLSRQFQ\nLNTPLLIQDINTIKDALDGILQREFQLSEKVEPAAIALSGDADLWLGNLVRQIFASHSGY\nDVNALALDAEIESDLGIDSVSVASAQGELFNTLQLNSETIIANCNTLSALKQCLAARLVQ\nEKGQDWFEQRGRGQSDSAIDHDADTTAEVTPPTATPVAINAEIGDPRTMRDFVGIEHPDI\nFHKAREFHLFYQDKKKRQLYFYGMPLETPCKNRAVMFDEATGQHREFLMFGSNSYLGLSN\nHPEIIHAIQDAASLYGATNTGCRIIAGSNVLHLELERKLAKLKGRDDCIVYPSGYSANLG\nCISALTSRHDLVFTDAINHMSIQDGCKLAGAQRKIYNHSLTSLEKSLAKYADHPGGKLIV\nTDGVFSMHGDIVDLPRLMKLAERYGARVLVDDAHSTGVLGKTGAGTSEHFNMKGQVDLEL\nGTMSKALSGLGGYVCGDGDVVEYLRFYSNSYVFAATIPAPVAAGVIASIDVMLREPERLA\nKLWDNIYYFRTRLLNAGFDLENSDSAIIPIVVGDDAKTLFFGRAVRARGMFCQTVVFPGV\nSVGDARLRISITSEHTREDLDEAYAILVASALEVGVPVNASAHQEENASVAEA","mw":22.209,"total_mw":22.209,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris supplemented with 5 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Di-domain acyl carrier protein of the seryltransferase from prodigiosin biosynthesis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Thailand","full_name":"Kasetsart University","webpage":"https://www.ku.ac.th"}],"contributor_name":"Pakorn","contributor_surname":"Wattana-Amorn","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-01-13","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":600.0,"number_of_frames":1,"wavelength":0.131,"sample_detector_distance":0.85,"concentration_min":1.7,"concentration_max":6.8,"sample_volume":null,"flow_rate":null,"s_min":0.411,"s_max":3.612,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDH6_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"r6669","model_data":"https://www.sasbdb.org/media/pdb_file/SASDDH6_fit1_model1.pdb","model_mw":22.5,"bead_radius":1.8,"log":null,"symmetry":"P1","comment":null,"user":318},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDH6_fit1_model2_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5","model_data":"https://www.sasbdb.org/media/pdb_file/SASDDH6_fit1_model2.pdb","model_mw":22.461,"bead_radius":2.0,"log":null,"symmetry":null,"comment":null,"user":318}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDH6_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":2.864,"p_value":0.3102,"fit_residual_plot":"SASDDH6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDH6_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/run_1_alDHk3F.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":"Two DAMMIF-derived dummy atom models are displayed. Top: The best-fit individual DAMMIF model and corresponding fit to the SAXS data. Bottom: The average spatial representation of the protein obtained after the alignment of multiple individual DAMMIF models combined with volume and bead-occupancy corrections (damfilt).","experiment_description":"Synchrotron SAXS data from solutions of the di-domain acyl carrier protein of the seryltransferase from prodigiosin biosynthesis in 20 mM Tris, 5 mM DTT, pH 7, were collected on the BL1.3W beam line at the Synchrotron Light Research Institute (Nakhon Ratchasima, Thailand) using a CCD Rayonix SX165 detector at a sample-detector distance of 0.9 m and at a wavelength of λ = 0.131 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.7 and 6.8 mg/ml were measured at 25°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":19.107,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":2.343e-05,"pddf_i0_error":3.811e-06,"guinier_i0":2.2764e-05,"guinier_i0_error":1.6e-06,"pddf_rg":2.56,"pddf_rg_error":0.36,"guinier_rg":2.39,"guinier_rg_error":0.18,"pddf_dmax":8.43,"pddf_dmax_error":null,"porod_volume":39.44,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":11,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDH6_datloglog_img.png","symmetry":null,"last_modified":"2024-10-17T15:41:17.447524+02:00","bragg_peak":[]}