{"code":"SASDDH9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"<i>Arabidopsis</i> and <i>Chlamydomonas</i> phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle.","publication":{"title":"<i>Arabidopsis</i> and <i>Chlamydomonas</i> phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin-Benson cycle.","author_list":"Gurrieri L, Del Giudice A, Demitri N, Falini G, Pavel NV, Zaffagnini M, Polentarutti M, Crozet P, Marchand CH, Henri J, Trost P, Lemaire SD, Sparla F, Fermani S","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1820639116","pmid":"30923119","published_date":"2019 Apr 16"},"status":"released","submitted_date":"2018-07-05","released_date":"2019-03-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDH9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDH9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDH9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDH9.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Phosphoribulokinase, chloroplastic","short_name":"CrPRK","sequence":"GSHMDKDKTVVIGLAADSGCGKSTFMRRMTSIFGGVPKPPAGG\r\nNPDSNTLISDMTTVICLDDYHCLDRNGRKVKGVTALAPEAQNFDLMYNQVKALKEGKSVDKPIYNHVSGL\r\nIDAPEKIESPPILVIEGLHPFYDKRVAELLDFKIYLDISDDIKFAWKIQRDMAERGHSLESIKSSIAARK\r\nPDFDAYIDPQKKDADMIIQVLPTQLVPDDKGQYLRVRLIMKEGSKMFDPVYLFDEGSTISWIPCGRKLTC\r\nSFPGIKMFYGPDTWYGQEVSVLEMDGQFDKLEELIYVESHLSNTSAKFYGEITQQMLKNSGFPGSNNGTG\r\nLFQTIVGLKVREVYERIVKKDVVPV","organism":"Chlamydomonas reinhardtii","uniprot_code":"P19824","uniprot_range_first":32,"uniprot_range_last":375,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MAFTMRAPAPRATAQSRVTANRARRSLVVRADKDKTVVIGLAADSGCGKSTFMRRMTSIF\nGGVPKPPAGGNPDSNTLISDMTTVICLDDYHCLDRNGRKVKGVTALAPEAQNFDLMYNQV\nKALKEGKSVDKPIYNHVSGLIDAPEKIESPPILVIEGLHPFYDKRVAELLDFKIYLDISD\nDIKFAWKIQRDMAERGHSLESIKSSIAARKPDFDAYIDPQKKDADMIIQVLPTQLVPDDK\nGQYLRVRLIMKEGSKMFDPVYLFDEGSTISWIPCGRKLTCSFPGIKMFYGPDTWYGQEVS\nVLEMDGQFDKLEELIYVESHLSNTSAKFYGEITQQMLKNSGFPGSNNGTGLFQTIVGLKV\nREVYERIVKKDVVPV","mw":38.908,"total_mw":77.816,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"Tris-HCl 50 mM 150 mM KCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Chloroplastic phosphoribulokinase (collected using SEC-SAXS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Sapienza","address":"Rome, Italy","full_name":"Sapienza University Rome","webpage":"http://www.uniroma1.it/"}],"contributor_name":"Alessandra","contributor_surname":"Del Giudice","orcid":"https://orcid.org/0000-0002-1916-8300"}],"concentration_method":null,"concentration_unit":null,"date":"2016-02-15","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":1.0,"number_of_frames":80,"wavelength":0.099,"sample_detector_distance":2.872,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.122,"s_max":3.502,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDH9_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6h7g","ext_code":"6H7G","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDH9_fit1_model1.pdb","model_mw":75.89,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":341}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/6h7g01_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.222,"p_value":2.1e-05,"fit_residual_plot":"6h7g01_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDH9_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/6h7g01.log","software_version":"CRYSOL_30","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Additional SEC parameters: Column type: Superdex 200 10/300 GL (GE Healthcare); Flow rate: 0.5 ml/min; Sample injection concentration: 6.1 mg/ml; Injection volume: 100 µl.","experiment_description":"Synchrotron SAXS data from solutions of chloroplastic phosphoribulokinase (collected using SEC-SAXS) in Tris-HCl 50 mM 150 mM KCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4°C. 80 successive 1 second frames were collected through the SEC-elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank (eluate SEC-buffer) was subtracted.","tags":[],"intensity_unit":null,"experimental_mw":70.0,"experimental_mw_error":null,"guinier_i0_mw":70.0,"guinier_i0_mw_error":0.2,"porod_mw":72.0,"porod_mw_error":null,"pddf_i0":42.42,"pddf_i0_error":0.06,"guinier_i0":42.0,"guinier_i0_error":0.1,"pddf_rg":3.548,"pddf_rg_error":0.008,"guinier_rg":3.43,"guinier_rg_error":0.01,"pddf_dmax":11.3,"pddf_dmax_error":null,"porod_volume":115.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":55,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDH9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}