{"code":"SASDDL6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The X-ray structure of human calbindin-D28K: an improved model.","publication":{"title":"The X-ray structure of human calbindin-D28K: an improved model.","author_list":"Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR","journal":"Acta Crystallogr D Struct Biol","doi":"10.1107/S2059798318011610","pmid":"30289411","published_date":"2018 Oct 1"},"status":"released","submitted_date":"2018-05-17","released_date":"2018-10-04"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDL6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDL6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDL6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDL6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDL6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDL6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDL6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Calbindin","short_name":"CALB1","sequence":"GPHMAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN","organism":"Homo sapiens","uniprot_code":"P05937","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKT\nFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIET\nEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLK\nFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMA\nLSDGGKLYRTDLALILCAGDN","mw":30.316,"total_mw":30.316,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 150 mM NaCl, 3 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.8,"deuteration":null},"purity_method":null,"name":"Calcium Loaded Calbindin-D28K","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Brighton, UK","full_name":"University of Sussex","webpage":"http://www.sussex.ac.uk/"}],"contributor_name":"James","contributor_surname":"Noble","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-28","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":3.0,"number_of_frames":15,"wavelength":0.1,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":11.5,"sample_volume":null,"flow_rate":null,"s_min":0.037,"s_max":3.696,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDL6_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6fie","ext_code":"6FIE","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDL6_fit1_model1.pdb","model_mw":28.58,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":280}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDL6_fit1_fitfoxs_img.png","software":"FoXS","chi_square_value":1.1878,"p_value":0.0,"fit_residual_plot":"SASDDL6_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDL6_fit1.dat","fit_log":null,"software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDL6_fit2_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"2g9b","ext_code":"2G9B","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDL6_fit2_model1.pdb","model_mw":29.98,"bead_radius":null,"log":null,"symmetry":null,"comment":"modle 2 selected","user":280}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDL6_fit2_fitfoxs_img.png","software":"FoXS","chi_square_value":3.99164,"p_value":0.0,"fit_residual_plot":"SASDDL6_fit2_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDL6_fit2.dat","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of calcium-loaded calbindin-D28K in 20 mM Tris, 150 mM NaCl, 3 mM CaCl2, pH 7.8, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 11.50 mg/ml was measured at 25°C. 15 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":25.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":29.0,"porod_mw_error":null,"pddf_i0":0.08278,"pddf_i0_error":null,"guinier_i0":0.08262,"guinier_i0_error":0.0001,"pddf_rg":2.15,"pddf_rg_error":null,"guinier_rg":2.142,"guinier_rg_error":0.005,"pddf_dmax":7.28,"pddf_dmax_error":null,"porod_volume":47.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":33,"guinier_point_last":259,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDL6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}