{"code":"SASDDM3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.","publication":{"title":"Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.","author_list":"Franke D, Jeffries CM, Svergun DI","journal":"Biophys J","doi":"10.1016/j.bpj.2018.04.018","pmid":"29874600","published_date":"2018 Jun 5"},"status":"released","submitted_date":null,"released_date":"2018-06-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDM3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDM3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDM3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDM3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ribonuclease pancreatic","short_name":"RNase","sequence":">sp|P61823|RNAS1_BOVIN Ribonuclease pancreatic OS=Bos taurus GN=RNASE1 PE=1 SV=1\nMALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRN\nLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPN\nCAYKTTQANKHIIVACEGNPYVPVHFDASV\n\n","organism":"Bos taurus","uniprot_code":"P61823","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRN\nLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPN\nCAYKTTQANKHIIVACEGNPYVPVHFDASV","mw":16.46,"total_mw":16.46,"number_molecules":1,"complex_state":null,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":1.0,"deuteration":null},"purity_method":null,"name":"Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Cy M","contributor_surname":"Jeffries","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2013-07-29","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.03,"number_of_frames":17,"wavelength":0.124,"sample_detector_distance":3.1,"concentration_min":null,"concentration_max":5.97,"sample_volume":null,"flow_rate":null,"s_min":0.054,"s_max":3.495,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":"Included in the additional files of the full-entry zip archive are SAXS data measured from folded RNAse (rnase.dat - also refer to SASBDB entry SASDDL3) as well as unfolded carboyxamidomethylated ribonuclease A (rnaseU.dat) and unfolded RNAse in the presence of 1 M urea (rnaseUU1.dat) or 2 M urea (rnaseUU2.dat).","experiment_description":"Synchrotron SAXS data from solutions of carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea in 10 mM HCl, pH 1, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.97 mg/ml was measured at 10°C. 17 successive 0.030 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":13.4,"experimental_mw_error":null,"guinier_i0_mw":13.4,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":498.2,"pddf_i0_error":null,"guinier_i0":484.7,"guinier_i0_error":5.04,"pddf_rg":2.55,"pddf_rg_error":null,"guinier_rg":2.31,"guinier_rg_error":0.03,"pddf_dmax":9.0,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":154,"guinier_point_last":254,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDM3_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}