{"code":"SASDDN2","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function.","publication":{"title":"Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function.","author_list":"Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ","journal":"J Biol Chem","doi":"10.1074/jbc.M116.756965","pmid":"27679491","published_date":"2016 Nov 11"},"status":"released","submitted_date":"2018-01-09","released_date":"2018-01-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDN2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDN2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDN2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDN2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"MAR 165 CCD","resolution":null},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Sinorhizobium meliloti (SmPutA)","short_name":"SmPutA","sequence":"SMMSPNPLQKPAIDAAPAPFADFAPPVRPQSTLRRAITAAYRRPETECLPPLVEAATQSKEIRDAAASTARKLIEALRGK\r\nHSGSGVEGLVQEYSLSSQEGVALMCLAEALLRIPDTATRDALIRDKIADGNWKSHLGGSRSLFVNAATWGLVVTGKLTST\r\nVNDRSLAAALTRLISRCGEPVIRRGVDMAMRMMGEQFVTGETIREALKRSKELEEKGFSYSYDMLGEAATTAADAERYYR\r\nDYESAIHAIGKASAGRGIYEGPGISIKLSALHPRYSRAQAARVMGELLPRVKALALLAKNYDIGLNIDAEEADRLELSLD\r\nLLEVLCLDGDLSGWNGMGFVVQAYGKRCPFVLDFIIDLARRSGRRIMVRLVKGAYWDAEIKRAQLDGLADFPVFTRKIHT\r\nDVSYIACAAKLLAATDVVFPQFATHNAQTLAAIYHMAGKDFHVGKYEFQCLHGMGEPLYEEVVGRGKLDRPCRIYAPVGT\r\nHETLLAYLVRRLLENGANSSFVHRINDPKVSIDELIADPVEVVRAMPVVGAKHDRIALPAELFGDARTNSAGLDLSNEET\r\nLASLTEALRESAAMKWTALPQLATGPAAGETRTVLNPGDHRDVVGSVTETSEEDARRAVRLAADAAPDWAAVPPSERAAC\r\nLDRAAELMQARMPTLLGLIIREAGKSALNAIAEVREAIDFLRYYAEQTRRTLGPGHGPLGPIVCISPWNFPLAIFTGQIA\r\nAALVAGNPVLAKPAEETPLIAAEGVRILREAGIPASALQLLPGDGRVGAALVAAAETAGVMFTGSTEVARLIQAQLADRL\r\nSPAGRPIPLIAETGGQNAMIVDSSALAEQVVGDVITSAFDSAGQRCSALRVLCLQEDVADRILTMLKGALHELHIGRTDR\r\nLSVDVGPVITSEAKDNIEKHIERMRGLGRKVEQIGLASETGVGTFVPPTIIELEKLSDLQREVFGPVLHVIRYRRDDLDR\r\nLVDDVNATGYGLTFGLHTRLDETIAHVTSRIKAGNLYINRNIIGAVVGVQPFGGRGLSGTGPKAGGPLYLGRLVTTAPVP\r\nPQHSSVHTDPVLLDFAKWLDGKGARAEAEAARNAGSSSALGLDLELPGPVGERNLYTLHARGRILLVPATESGLYHQLAA\r\nALATGNSVAIDAASGLQASLKNLPQTVGLRVSWSKDWAADGPFAGALVEGDAERIRAVNKAIAALPGPLLLVQAASSGEI\r\nARNPDAYCLNWLVEEVSASINTAAAGGNASLMAIG","organism":"Sinorhizobium meliloti","uniprot_code":"F7X6I3","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSPNPLQKPAIDAAPAPFADFAPPVRPQSTLRRAITAAYRRPETECLPPLVEAATQSKEI\nRDAAASTARKLIEALRGKHSGSGVEGLVQEYSLSSQEGVALMCLAEALLRIPDTATRDAL\nIRDKIADGNWKSHLGGSRSLFVNAATWGLVVTGKLTSTVNDRSLAAALTRLISRCGEPVI\nRRGVDMAMRMMGEQFVTGETIREALKRSKELEEKGFSYSYDMLGEAATTAADAERYYRDY\nESAIHAIGKASAGRGIYEGPGISIKLSALHPRYSRAQAARVMGELLPRVKALALLAKNYD\nIGLNIDAEEADRLELSLDLLEVLCLDGDLSGWNGMGFVVQAYGKRCPFVLDFIIDLARRS\nGRRIMVRLVKGAYWDAEIKRAQLDGLADFPVFTRKIHTDVSYIACAAKLLAATDVVFPQF\nATHNAQTLAAIYHMAGKDFHVGKYEFQCLHGMGEPLYEEVVGRGKLDRPCRIYAPVGTHE\nTLLAYLVRRLLENGANSSFVHRINDPKVSIDELIADPVEVVRAMPVVGAKHDRIALPAEL\nFGDARTNSAGLDLSNEETLASLTEALRESAAMKWTALPQLATGPAAGETRTVLNPGDHRD\nVVGSVTETSEEDARRAVRLAADAAPDWAAVPPSERAACLDRAAELMQARMPTLLGLIIRE\nAGKSALNAIAEVREAIDFLRYYAEQTRRTLGPGHGPLGPIVCISPWNFPLAIFTGQIAAA\nLVAGNPVLAKPAEETPLIAAEGVRILREAGIPASALQLLPGDGRVGAALVAAAETAGVMF\nTGSTEVARLIQAQLADRLSPAGRPIPLIAETGGQNAMIVDSSALAEQVVGDVITSAFDSA\nGQRCSALRVLCLQEDVADRILTMLKGALHELHIGRTDRLSVDVGPVITSEAKDNIEKHIE\nRMRGLGRKVEQIGLASETGVGTFVPPTIIELEKLSDLQREVFGPVLHVIRYRRDDLDRLV\nDDVNATGYGLTFGLHTRLDETIAHVTSRIKAGNLYINRNIIGAVVGVQPFGGRGLSGTGP\nKAGGPLYLGRLVTTAPVPPQHSSVHTDPVLLDFAKWLDGKGARAEAEAARNAGSSSALGL\nDLELPGPVGERNLYTLHARGRILLVPATESGLYHQLAAALATGNSVAIDAASGLQASLKN\nLPQTVGLRVSWSKDWAADGPFAGALVEGDAERIRAVNKAIAALPGPLLLVQAASSGEIAR\nNPDAYCLNWLVEEVSASINTAAAGGNASLMAIG","mw":131.805,"total_mw":131.805,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP, and 50 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.8,"deuteration":null},"purity_method":null,"name":"Sinorhizobium meliloti Proline Utilization A (PutA) at 3.00 mg/ml","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"Mizzou","address":"Columbia, MO, USA","full_name":"University of Missouri-Columbia","webpage":"http://missouri.edu/"}],"contributor_name":"John","contributor_surname":"Tanner","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-03-27","storage_temperature":null,"cell_temperature":10.0,"exposure_time":null,"number_of_frames":null,"wavelength":0.1127,"sample_detector_distance":null,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.131,"s_max":3.256,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDN2_fit1_model1_img.png","software":"MES-FoXS","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDN2_fit1_model1.pdb","model_mw":127.7,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":166},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDN2_fit1_model2_img.png","software":"MES-FoXS","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDN2_fit1_model2.pdb","model_mw":254.1,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":166}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDN2_fit1_fitfoxs_img.png","software":"MES-FoXS","chi_square_value":1.37025,"p_value":0.0,"fit_residual_plot":"SASDDN2_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDN2_fit1.dat","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The fit to the SAXS data is shown for a volume-fraction weighted mixture of monomers and dimers. The volume fraction of monomer and dimer are 0.67 and 0.33, respectively.","experiment_description":"Synchrotron SAXS data from solutions of Sinorhizobium meliloti proline utilization A (PutA) in 50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP and 50 mM NaCl, pH 7.8, were collected on the 12.3.1 SIBYLS beam line at the Advanced Light Source storage ring (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":181.67,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":1548.0,"pddf_i0_error":4.3,"guinier_i0":1566.68,"guinier_i0_error":12.0,"pddf_rg":3.86,"pddf_rg_error":null,"guinier_rg":3.842,"guinier_rg_error":0.04,"pddf_dmax":11.81,"pddf_dmax_error":null,"porod_volume":248.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":35,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDN2_datloglog_img.png","symmetry":null,"last_modified":"2026-05-18T10:05:51.027670+02:00","bragg_peak":[]}