{"code":"SASDDN6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding.","publication":{"title":"Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding.","author_list":"Trcka F, Durech M, Vankova P, Chmelik J, Martinkova V, Hausner J, Kadek A, Marcoux J, Klumpler T, Vojtesek B, Muller P, Man P","journal":"Mol Cell Proteomics","doi":"10.1074/mcp.RA118.001044","pmid":"30459217","published_date":"2018 Nov 20"},"status":"released","submitted_date":"2018-05-17","released_date":"2018-11-30"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDN6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDN6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDN6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDN6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"CEITEC","city":"Brno","country":"Czech Republic","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":"","type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Heat shock 70 kDa protein 1","short_name":"HSPA1A_T204A","sequence":"MSYYHHHHHHLESTSLYKKAGFGSENLYFQSMAKAAAIGI\r\nDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER\r\nLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKH\r\nWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKE\r\nIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNV\r\nLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGAFDVSI\r\nLTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKH\r\nKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFE\r\nGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKA\r\nQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAV\r\nAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGV\r\nMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAM\r\nTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVT\r\nATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAED\r\nEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKK\r\nVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISG\r\nLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD","organism":"Homo sapiens","uniprot_code":"P0DMV8","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA\nLNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEIS\nSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAA\nIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNH\nFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRA\nRFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLN\nKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTI\nPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDI\nDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKN\nALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE\nQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD","mw":73.74,"total_mw":147.48,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50mM HEPES, 150mM KCH3COO, 2mM MgCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Human stress-inducible heat shock 70 kDa protein 1, Hsp70 (Hspa1a) T204A mutant dimer, in the presence of ATP","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"CEITEC - Central European Institute of Technology, Masaryk University","webpage":"https://www.ceitec.eu/"}],"contributor_name":"Tomas","contributor_surname":"Klumpler","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-20","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":3600.0,"number_of_frames":null,"wavelength":0.154,"sample_detector_distance":0.48,"concentration_min":null,"concentration_max":2.5,"sample_volume":null,"flow_rate":null,"s_min":0.138,"s_max":6.314,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDN6_fit1_model1_img.png","software":"MODELLER","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDN6_fit1_model1.pdb","model_mw":148.5,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":195}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDN6_fit1_fit_img.png","software":"CRYSOL","chi_square_value":1.15,"p_value":0.489949,"fit_residual_plot":"SASDDN6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDN6_fit1.fit","fit_log":null,"software_version":"2.8.3","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of human stress-induced heat shock 70 kDa protein 1 ,Hsp70, (Hspa1a T204A mutant), with ATP in 50mM HEPES; 150mM KCH3COO; 2mM MgCl2, pH 7.5, were collected on a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":148.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":155.0,"porod_mw_error":null,"pddf_i0":0.7457,"pddf_i0_error":0.0058,"guinier_i0":0.76,"guinier_i0_error":0.011,"pddf_rg":3.97,"pddf_rg_error":0.2,"guinier_rg":4.01,"guinier_rg_error":0.077,"pddf_dmax":11.56,"pddf_dmax_error":null,"porod_volume":248.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":14,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDN6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}