{"code":"SASDDP6","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering.","publication":{"title":"Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering.","author_list":"Sutton EJ, Bradshaw RT, Orr CM, Frendéus B, Larsson G, Teige I, Cragg MS, Tews I, Essex JW","journal":"Biophys J","doi":"10.1016/j.bpj.2018.03.040","pmid":"30021105","published_date":"2018 Jul 17"},"status":"released","submitted_date":"2018-05-18","released_date":"2019-05-09"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDP6.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDP6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDP6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDP6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDP6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDP6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDP6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment","short_name":"6G08 F(ab)","sequence":"EVQLLESGGGLVQPGGSLRLSCAASGFTLSSYGISWVRQAPGKGLEWVSGISGSGGNTYYADSVKGRFTISRDNSKNTLY\r\nLQMNSLRAEDTAVYYCASSVGAYANDAFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTV\r\nSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK\r\nQSVLTQPPSASGTPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYGDTNRPSGVPDRFSGSKSGTSASLAISGL\r\nRSEDEADYYCAAWDDSLNGPVFGGGTKLTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPV\r\nKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":45.855,"total_mw":45.855,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50mM HEPES, 150mM KCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment (6G08 F(ab))","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Southampton, UK","full_name":"University of Southampton","webpage":"https://www.southampton.ac.uk/"}],"contributor_name":"Emma","contributor_surname":"Sutton","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2014-12-05","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":2.0,"number_of_frames":10,"wavelength":0.099,"sample_detector_distance":null,"concentration_min":1.25,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.141,"s_max":4.771,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ScÅtter","pddf_software_version":"3.0","i0_calibration_standard":null,"description":"Sample concentration series: 1.25mg/ml, 2.5mg/ml and 5mg/ml.","experiment_description":"Synchrotron SAXS data from solutions of Anti-CD32b Antibody Clone 6G08 Antibody Binding Fragment (6G08 F(ab)) in 50mM HEPES, 150mM KCl, pH 7.5, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 5 mg/ml were measured at 20°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower sample concentrations were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":44.4,"experimental_mw_error":null,"guinier_i0_mw":44.0,"guinier_i0_mw_error":null,"porod_mw":40.85,"porod_mw_error":null,"pddf_i0":42.43,"pddf_i0_error":0.09977,"guinier_i0":44.38,"guinier_i0_error":0.0225,"pddf_rg":2.54,"pddf_rg_error":0.0,"guinier_rg":2.55,"guinier_rg_error":0.02,"pddf_dmax":7.3,"pddf_dmax_error":null,"porod_volume":67.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":78,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDP6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}