{"code":"SASDDQ6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.","publication":{"title":"FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.","author_list":"Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T","journal":"PLoS Biol","doi":"10.1371/journal.pbio.2006026","pmid":"30071011","published_date":"2018 Aug"},"status":"released","submitted_date":"2018-05-19","released_date":"2018-06-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDQ6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDQ6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDQ6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDQ6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDQ6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ferredoxin Protease","short_name":"FusC","sequence":"EEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDIRLIVDVGSIDEKDNESGVAH\r\nMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYERTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERK\r\nIILEEWRGKLGVAERMNQQRVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI\r\nQRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRHRLLTQITMSAVTRQVRRQKA\r\nELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISAVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETRE\r\nFADWVQQLTIVWQQDRPYVGSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW\r\nALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGKKVYLTATSQAGFMATAMNPW\r\nQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEADQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMS\r\nLARQKANDDQSVGGKRASEMTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA\r\nTIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIASKYLKTSLRDDALGIYRMRVD\r\nSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTKITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYND\r\nPRYLSNASKLADSITLESVRAMSAKLYNPDNRVLYITLPQEVKE","organism":"Pectobacterium atrosepticum SCRI1043","uniprot_code":"Q6D8U3","uniprot_range_first":26,"uniprot_range_last":924,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNWKKCCWLSLTAVGLLAGSVVSQAEEIKSPLPVFKEGTLANGFRYTLVQLEGPKTRVDI\nRLIVDVGSIDEKDNESGVAHMVEHMVFRASDAFPQGVSTELHKQGWGRGQSYNAVTNYER\nTMYMMSPPKGNLDLGATLQALSQMTGHAKLLQSDLDDERKIILEEWRGKLGVAERMNQQR\nVQAIRHDSRYPSRPVIGTEESINDTPASVLQDFYQRWYHPSNMRLMIIGDITPADAEREI\nQRYFAALPNVAVPTRDYYEPLLKPQLKVARLQDSQSGSSQVSFVYRFNDKDAFGQSEYRH\nRLLTQITMSAVTRQVRRQKAELPQDASSLVVRKSDIGKTTAALGFFANVMPGGHDAAISA\nVLKEIERFKRYPLNEQDITEITSDIREVAQRMSVTPETREFADWVQQLTIVWQQDRPYVG\nSQQRGKDALEALDTIKGEDVNRHWQRWLASPDTLAQFSVPGATPFTLPKPDAISKLQKQW\nALATLAPLRLEEKKIIPELPSVTQSGKRTAVKTFAAQKVEQWQLSNGDRVVWLRAPEAGK\nKVYLTATSQAGFMATAMNPWQAQLASQLVNQSGPATWSGESLSNWKKEKTLSLSIDQEAD\nQLTLSGTAPTEQLASLFGLYRELNVAPGIDPDVMKESMMSLARQKANDDQSVGGKRASEM\nTKLRFGEPAWQQPEIAELKKISAPALLSQWHKAASAPVTYYLIADMPATQLLPQVERYLA\nTIPRQPASEVKQHLALSGKREATSAINVEPRADILTWSFTPHAWTPQAAVQVSIARNIAS\nKYLKTSLRDDALGIYRMRVDSELEDKKQRIETEVSFTSAPERAQELWTLAEQAFSELPTK\nITQQDVDEQKAQFIRAEKGRQGDLTTIQRRLILSYRHYNDPRYLSNASKLADSITLESVR\nAMSAKLYNPDNRVLYITLPQEVKE","mw":101.253,"total_mw":101.253,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.8,"deuteration":null},"purity_method":null,"name":"The ferredoxin protease, FusC","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Melbourne, Australia","full_name":"Monash University","webpage":"http://www.monash.edu/"}],"contributor_name":"Grinter","contributor_surname":"Grinter","orcid":"https://orcid.org/0000-0002-8195-5348"}],"concentration_method":null,"concentration_unit":null,"date":"2017-04-06","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":null,"number_of_frames":null,"wavelength":0.103,"sample_detector_distance":2.68,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.064,"s_max":2.79,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Additional SEC parameters:\r\nColumn type: GE Healthcare Superdex S200 10/300; Flow rate: 0.4 ml/min; Sample injection concentration: 10 mg/ml; Injection volume: 100 µl.","experiment_description":"Synchrotron SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected from a sample of ferredoxin protease, FusC, using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) at the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia). Data were collected a using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm. The SEC mobile phase consisted of 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % v/v glycerol, pH 7.8, (20°C). The SAXS data data measured from the SEC-elution (sample peak and buffer) were normalized to the intensity of the transmitted beam and radially averaged. The data from the sample SEC-peak were scaled and averaged and the scattering of the solvent-blank was subtracted from the sample frames to produce the data displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":100.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":89.2,"porod_mw_error":null,"pddf_i0":0.1299,"pddf_i0_error":null,"guinier_i0":0.13,"guinier_i0_error":0.00011,"pddf_rg":3.74,"pddf_rg_error":null,"guinier_rg":3.68,"guinier_rg_error":0.013,"pddf_dmax":12.84,"pddf_dmax_error":null,"porod_volume":151.6,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":9,"guinier_point_last":74,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDQ6_datloglog_img.png","symmetry":null,"last_modified":"2020-04-22T08:28:46.473948+02:00","bragg_peak":[]}