{"code":"SASDDR3","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/A","project":{"title":"Structure of tRNA methyltransferase complex of Trm7 and Trm734 reveals a novel binding interface for tRNA recognition.","publication":{"title":"Structure of tRNA methyltransferase complex of Trm7 and Trm734 reveals a novel binding interface for tRNA recognition.","author_list":"Hirata A, Okada K, Yoshii K, Shiraishi H, Saijo S, Yonezawa K, Shimizu N, Hori H","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkz856","pmid":"31586407","published_date":"2019 Oct 5"},"status":"released","submitted_date":"2018-02-27","released_date":"2019-10-06"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDR3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDR3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDR3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDR3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDR3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDR3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDR3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 2M","resolution":0.172},"name":"Photon Factory (PF), High Energy Accelerator Research Organization 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mM HEPES, 200 mM KCl, 5% v/v Glycerol, 10mM β-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex from Sacharomyces cerevisiae","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"KEK","address":"Tsukuba, Japan","full_name":"High Energy Accelerater Research Organization (KEK)","webpage":"https://www.kek.jp"}],"contributor_name":"Kento","contributor_surname":"Yonezawa","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-12-16","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":20.0,"number_of_frames":40,"wavelength":0.1,"sample_detector_distance":2.05,"concentration_min":0.47,"concentration_max":3.1,"sample_volume":null,"flow_rate":null,"s_min":0.063,"s_max":4.099,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDR3_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDR3_fit1_model1.pdb","model_mw":141.44,"bead_radius":4.25,"log":"https://www.sasbdb.org/media/log_files/zc_001_xuZHpHZ.log","symmetry":"P1","comment":null,"user":275}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDR3_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.917,"p_value":0.034809,"fit_residual_plot":"SASDDR3_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDR3_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/zc_001_cMEHJMB.log","software_version":"ATSAS 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GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex in 50 mM HEPES-KOH, 200 mM, KCl, 5 % v/v glycerol, 10 mM β-mercaptoethanol, pH 8.0 were collected on the beam line BL-10C at the Phton Factory (PF; Tsukuba, Ibaraki, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.05 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 40 images of 20 second frames were collected at 20°C. The data were normalized to the intensity of the incident beam and circularly averaged; the scattering of the solvent-blank was subtracted. The extrapolated scattering profile at a concentration of zero was calculated PRIMUS QT of ATSAS.","tags":[],"intensity_unit":"1/cm","experimental_mw":158.819,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":136.25,"porod_mw_error":null,"pddf_i0":0.1921,"pddf_i0_error":0.0001,"guinier_i0":0.192981,"guinier_i0_error":0.0001,"pddf_rg":3.77,"pddf_rg_error":0.003,"guinier_rg":3.79,"guinier_rg_error":0.003,"pddf_dmax":12.99,"pddf_dmax_error":null,"porod_volume":218.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":7,"guinier_point_last":54,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDR3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}