{"code":"SASDDS4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"An N-terminally truncated form of cyclic GMP-dependent protein kinase Iα (PKG Iα) is monomeric and autoinhibited and provides a model for activation.","publication":{"title":"An N-terminally truncated form of cyclic GMP-dependent protein kinase Iα (PKG Iα) is monomeric and autoinhibited and provides a model for activation.","author_list":"Moon TM, Sheehe JL, Nukareddy P, Nausch LW, Wohlfahrt J, Matthews DE, Blumenthal DK, Dostmann WR","journal":"J Biol Chem","doi":"10.1074/jbc.RA117.000647","pmid":"29602907","published_date":"2018 May 25"},"status":"released","submitted_date":"2018-03-02","released_date":"2018-03-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDS4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDS4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDS4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDS4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDS4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDS4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDS4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Rayonix MX225-HE","resolution":null},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"cGMP-dependent protein kinase 1","short_name":"∆53 PKG Iα","sequence":"GAMDIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF","organism":"Bos taurus","uniprot_code":"P00516","uniprot_range_first":54,"uniprot_range_last":671,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTR\nAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMY\nPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTAT\nVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEET\nHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDV\nRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDF\nNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDF\nIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGI\nIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISAD\nYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPS\nERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPP\nPDDNSGWDIDF","mw":70.433,"total_mw":70.433,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM MES, 300 mM NaCl, 1 mM TCEP, 5 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.9,"deuteration":null},"purity_method":null,"name":"cGMP-dependent protein kinase 1: ∆53 PKG Iα","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Tucson, AZ, USA","full_name":"University of Arizona","webpage":"http://www.arizona.edu/"}],"contributor_name":"Thomas","contributor_surname":"Moon","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-06-06","storage_temperature":4.0,"cell_temperature":22.0,"exposure_time":1.0,"number_of_frames":600,"wavelength":0.1127,"sample_detector_distance":1.7,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.087,"s_max":5.136,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDS4_fit1_model1_img.png","software":"DAMFILT","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"5.0","model_data":"https://www.sasbdb.org/media/pdb_file/SASDDS4_fit1_model1.pdb","model_mw":73.548,"bead_radius":3.25,"log":null,"symmetry":"p1","comment":"Filtered, Averaged 3-D envelope","user":268}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDS4_fit1_fit_img.png","software":"DAMMIN","chi_square_value":0.602,"p_value":0.999,"fit_residual_plot":"SASDDS4_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDS4_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/DAMMINon_102.log","software_version":"online","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of cGMP-dependent protein kinase 1 (∆53 PKG Iα) were collected using SEC-SAXS on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A 35 μL sample of 6 mg/mL PKG Iα ∆53 was injected onto a Superdex 200 3.2/30 column (GE) and eluted isocratically using an Ettan FPLC (GE) at 0.05 mL/min in 50 mM MES, 300 mM NaCl, 1mM TCEP, pH 6.9 supplemented with 5 mM DTT as a radical scavenger. The eluant stream was connected in-line to a 1.5 mm quartz capillary. Data were collected with a 1 second/frame exposure rate at 22°C. A total of 600 frames were collected. Frames 410-459 containing the peak corresponding to ∆53 PKG were averaged and subtracted from background. The scattering curve, Guinier and P(r) functions, and Porod volume were calculated using the PRIMUS (ATSAS) program suite.","tags":[],"intensity_unit":"arbitrary","experimental_mw":70.433,"experimental_mw_error":null,"guinier_i0_mw":75.814,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":323.9,"pddf_i0_error":1.8,"guinier_i0":321.37,"guinier_i0_error":2.21,"pddf_rg":3.02,"pddf_rg_error":0.03,"guinier_rg":2.971,"guinier_rg_error":0.031,"pddf_dmax":9.68,"pddf_dmax_error":null,"porod_volume":105.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":10,"guinier_point_last":39,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDS4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}