{"code":"SASDDT5","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Stability of local secondary structure determines selectivity of viral RNA chaperones.","publication":{"title":"Stability of local secondary structure determines selectivity of viral RNA chaperones.","author_list":"Bravo JPK, Borodavka A, Barth A, Calabrese AN, Mojzes P, Cockburn JJB, Lamb DC, Tuma R","journal":"Nucleic Acids Res","doi":"10.1093/nar/gky394","pmid":"29796667","published_date":"2018 May 18"},"status":"released","submitted_date":"2018-04-01","released_date":"2018-04-26"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDT5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDT5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDT5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDT5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nonstructural protein sigma NS","short_name":null,"sequence":"MDNTVRVGVSRNTSGAAGQTLFRNFYLLRCNISADGRNATKAVQSHFPFLSRAVRCLSPL\r\nAAHCADRTLRRDNVKQILTRELPFSSDLINYAHHVNSSSLTTSQGVEAARLVAQVYGEQV\r\nPFDHIYPTGSATYCPGAIANAISRIMAGFVPREGDDFAPSGPIDYLAADLIAYKFVLPYM\r\nLDMVDGRPQIVLPSHTVEEMLTNTSLLNSIDASFGIEARSDQRMTRDAAEMSSRSLNELE\r\nDHDQRGRMPWKIMLAMMAAQLKVELDALADERTESQANAHVTSFGSRLFNQMSAFVTIDR\r\nELMELALLIKEQGFAMNPGQIASKWSLIRRSGPTRPLSGARLEIRNGNWMIREGDQTLLS\r\nVSPARMA","organism":"Avian orthoreovirus","uniprot_code":"Q9DH28","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"octamer","molecular_type":"protein","uniprot_sequence":"MDNTVRVGVSRNTSGAAGQTLFRNFYLLRCNISADGRNATKAVQSHFPFLSRAVRCLSPL\nAAHCADRTLRRDNVKQILTRELPFSSDLINYAHHVNSSSLTTSQGVEAARLVAQVYGEQV\nPFDHIYPTGSATYCPGAIANAISRIMAGFVPREGDDFAPSGPIDYLAADLIAYKFVLPYM\nLDMVDGRPQIVLPSHTVEEMLTNTSLLNSIDASFGIEARSDQRMTRDAAEMSSRSLNELE\nDHDQRGRMPWKIMLAMMAAQLKVELDALADERTESQANAHVTSFGSRLFNQMSAFVTIDR\nELMELALLIKEQGFAMNPGQIASKWSLIRRSGPTRPLSGARLEIRNGNWMIREGDQTLLS\nVSPARMA","mw":40.586,"total_mw":324.688,"number_molecules":8,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"20mer RNA (unstructured)","short_name":"20mer","sequence":"CUUUUCAAGACAUGCAACAA","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"RNA","uniprot_sequence":null,"mw":6.399,"total_mw":12.798,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM HEPES, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Leeds, UK","full_name":"University of Leeds","webpage":"https://www.leeds.ac.uk/"}],"contributor_name":"Jack","contributor_surname":"Bravo","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-02-25","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":0.1,"sample_detector_distance":3.9,"concentration_min":null,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.037,"s_max":3.7,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SigmaNS ribonucleoprotein complex with 20mer RNA. Sample concentration, UNKNOWN; Sample temperature, UNKNOWN; Exposure time; UNKNOWN.","experiment_description":"Synchrotron SAXS data from solutions of ribonucleoprotein complex of nonstructural protein sigma NS bound to 20mer RNA (NS-RNP20) in 25 mM HEPES, 150 mM NaCl, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":323.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.3067,"pddf_i0_error":null,"guinier_i0":0.2991,"guinier_i0_error":0.001,"pddf_rg":8.65,"pddf_rg_error":null,"guinier_rg":7.82,"guinier_rg_error":0.06,"pddf_dmax":38.0,"pddf_dmax_error":null,"porod_volume":964.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":45,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDT5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}