{"code":"SASDDT8","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.","publication":{"title":"Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.","author_list":"Roed NK, Viola CM, Kristensen O, Schluckebier G, Norrman M, Sajid W, Wade JD, Andersen AS, Kristensen C, Ganderton TR, Turkenburg JP, De Meyts P, Brzozowski AM","journal":"Nat Commun","doi":"10.1038/s41467-018-06192-3","pmid":"30242155","published_date":"2018 Sep 21"},"status":"released","submitted_date":"2018-06-21","released_date":"2018-12-03"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDT8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDT8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDT8.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"ID14-3","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Insulin-like peptide 5","short_name":null,"sequence":"NSLRACGPALMDMLRVACPNGFNSMFAK\r\nGVVDSCCRKSCSFSTLRAYCDS","organism":"Drosophila melanogaster","uniprot_code":"E7BBS4","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MFRSVIPVLLFLIPLLLSAQAANSLRACGPALMDMLRVACPNGFNSMFAKRGTLGLFDYE\nDHLADLDSSESHHMNSLSSIRRDFRGVVDSCCRKSCSFSTLRAYCDS","mw":5.385,"total_mw":5.385,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Neural/ectodermal development factor IMP-L2","short_name":"Imp-L2 IBP","sequence":"MEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF\r\nTKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR\r\nVRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE\r\nKTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG\r\nNYKCIARNVVGKDTADTFVYPVLNEED","organism":"Drosophila melanogaster","uniprot_code":"Q09024","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEAKMNLHVCALALLLFGSIATVRGRAVDLVDDSNDVDNSIEAEEEKPRNRAFEADWLKF\nTKTPPTKLQQADGATIEIVCEMMGSQVPSIQWVVGHLPRSELDDLDSNQVAEEAPSAIVR\nVRSSHIIDHVLSEARTYTCVGRTGSKTIYASTVVHPPRSSRLTPEKTYPGAQKPRIIYTE\nKTHLDLMGSNIQLPCRVHARPRAEITWLNNENKEIVQGHRHRVLANGDLLISEIKWEDMG\nNYKCIARNVVGKDTADTFVYPVLNEED","mw":29.895,"total_mw":29.895,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"phosphate buffered saline","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Denmark","full_name":"Novo Nordisk A/S","webpage":"https://www.novonordisk.com/"}],"contributor_name":"Mathias","contributor_surname":"Norrman","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2011-11-20","storage_temperature":5.0,"cell_temperature":25.0,"exposure_time":10.0,"number_of_frames":10,"wavelength":0.093,"sample_detector_distance":2.43,"concentration_min":2.3,"concentration_max":9.3,"sample_volume":null,"flow_rate":null,"s_min":0.064,"s_max":6.108,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of neural/ectodermal development factor IMP-L2 in complex with insulin-like peptide 5 (DILP5) in phosphate buffered saline, pH 7.4 were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.093 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 9.3 mg/ml were measured at 25°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":30.0,"experimental_mw_error":null,"guinier_i0_mw":30.0,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":44.77,"guinier_i0_error":0.03,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":2.59,"guinier_rg_error":0.04,"pddf_dmax":9.0,"pddf_dmax_error":null,"porod_volume":55.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":26,"guinier_point_last":65,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDT8_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}