{"code":"SASDDV4","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Conformational Plasticity of the Immunoglobulin Fc Domain in Solution.","publication":{"title":"Conformational Plasticity of the Immunoglobulin Fc Domain in Solution.","author_list":"Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M","journal":"Structure","doi":"10.1016/j.str.2018.03.017","pmid":"29731233","published_date":"2018 Jul 3"},"status":"released","submitted_date":"2018-03-06","released_date":"2018-04-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDV4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDV4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDV4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDV4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDV4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDV4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDV4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Immunoglobulin heavy constant gamma 1 M255Y/S257T/T259E","short_name":"IgG1 Fc-YTE","sequence":"TCPPCPAPELLGGPSVFLFPPKPKDTLYITREPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPR\r\nEEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTK\r\nNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM\r\nHEALHNHYTQKSLSLSPGK","organism":"Homo sapiens","uniprot_code":"P01857","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS\nGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG\nPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYN\nSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDE\nLTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW\nQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","mw":26.6,"total_mw":53.2,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES, 50mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Fc-region of Immunoglobulin G1, M135Y/S137T/T139E mutant (IgG1 Fc-YTE)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"UCSF","address":"San Francisco, CA, USA","full_name":"University of California, San Francisco (UCSF)","webpage":"https://www.ucsf.edu/"}],"contributor_name":"Soumya","contributor_surname":"G Remesh","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2016-02-17","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":0.3,"number_of_frames":33,"wavelength":0.103,"sample_detector_distance":1.5,"concentration_min":1.0,"concentration_max":14.0,"sample_volume":null,"flow_rate":null,"s_min":0.109,"s_max":5.319,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDV4_fit1_model1_img.png","software":"BILBOMD","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDV4_fit1_model1.pdb","model_mw":211.3,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":293}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDV4_fit1_fitfoxs_img.png","software":"MultiFoXS","chi_square_value":1.58697,"p_value":0.0,"fit_residual_plot":"SASDDV4_fit1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDV4_fit1.dat","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the Fc-region of Immunoglobulin G1, M135Y/S137T/T139E mutant (IgG1 Fc-YTE) in 20 mM HEPES, 50mM NaCl, pH 7.5, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 14 mg/ml were measured at 20°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":53.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":499.7,"pddf_i0_error":1.0,"guinier_i0":497.0,"guinier_i0_error":1.4,"pddf_rg":2.75,"pddf_rg_error":0.006,"guinier_rg":2.73,"guinier_rg_error":0.01,"pddf_dmax":10.0,"pddf_dmax_error":0.01,"porod_volume":74.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":67,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDV4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}