{"code":"SASDDW4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.","publication":{"title":"Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.","author_list":"Scietti L, Chiapparino A, De Giorgi F, Fumagalli M, Khoriauli L, Nergadze S, Basu S, Olieric V, Cucca L, Banushi B, Profumo A, Giulotto E, Gissen P, Forneris F","journal":"Nat Commun","doi":"10.1038/s41467-018-05631-5","pmid":"30089812","published_date":"2018 Aug 8"},"status":"released","submitted_date":"2018-03-09","released_date":"2018-08-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDW4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDW4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDW4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDW4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDW4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDW4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDW4.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Procollagen lysyl hydroxylase LH3","short_name":"LH3","sequence":"SSDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTV\r\nGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFC\r\nWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGL\r\nREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNY\r\nLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYP\r\nPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPE\r\nCEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDY\r\nVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFL\r\nHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCP\r\nDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWL\r\nQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGL\r\nDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDPAAA","organism":"Homo sapiens","uniprot_code":"O60568","uniprot_range_first":25,"uniprot_range_last":738,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAE\nFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAG\nSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIV\nRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRI\nRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFL\nAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLV\nGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSR\nHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRD\nVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDW\nKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRL\nAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDE\nQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHE\nGLPTTWGTRYIMVSFVDP","mw":90.0,"total_mw":180.0,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM HEPES, 200 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Procollagen lysyl hydroxylase LH3 measured using SEC-SAXS","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Italy","full_name":"University of Pavia","webpage":"http://wcm-3.unipv.it"}],"contributor_name":"Federico","contributor_surname":"Forneris","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-28","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":75,"wavelength":0.09919,"sample_detector_distance":2.87,"concentration_min":null,"concentration_max":3.5,"sample_volume":null,"flow_rate":null,"s_min":0.051,"s_max":4.928,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDDW4_fit3_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDDW4_fit3_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":294}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDW4_fit3_fit_img.png","software":"GASBOR","chi_square_value":1.85,"p_value":0.0,"fit_residual_plot":"SASDDW4_fit3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDDW4_fit3.fir","fit_log":null,"software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The sample was injected at a load concentration of 3.5 mg/mL. The SEC column used was a Superdex 200 PC 3.2/300 Increase (GE Healthcare) at a flow rate 0.075 mL/min.","experiment_description":"Synchrotron SAXS data from solutions of procollagen lysyl hydroxylase LH3 in 25 mM HEPES, 200 mM NaCl, pH 8 were measured at 20°C using SEC-SAXS collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) equipped with a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was injected onto the column. 75 successive 1 second frames were collected through the sample elution peak and processed using CHROMIXS that included the identification and subtraction of an appropriate solvent-blank.","tags":[],"intensity_unit":"arbitrary","experimental_mw":180.0,"experimental_mw_error":null,"guinier_i0_mw":184.0,"guinier_i0_mw_error":6.0,"porod_mw":170.0,"porod_mw_error":null,"pddf_i0":62.4,"pddf_i0_error":0.1,"guinier_i0":61.5,"guinier_i0_error":0.12,"pddf_rg":5.346,"pddf_rg_error":0.02,"guinier_rg":5.05,"guinier_rg_error":0.07,"pddf_dmax":20.96,"pddf_dmax_error":null,"porod_volume":268.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":9,"guinier_point_last":43,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDW4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}