{"code":"SASDDX4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.","publication":{"title":"A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.","author_list":"Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J","journal":"ACS Comb Sci","doi":"10.1021/acscombsci.8b00007","pmid":"29553252","published_date":"2018 Apr 9"},"status":"released","submitted_date":"2018-03-15","released_date":"2018-03-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDDX4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDDX4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDX4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDX4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDX4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDX4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDDX4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Protein sex-lethal","short_name":"Sxl","sequence":">P19339 Sxl-wildtype 123-294 with GAMA cleavage site\r\nGAMASNTNLIVNYLPQDMTDRELYALFRAIGPINTCRIMRDYKTGYSFGYAFVDFTSEMDSQ\r\nRAIKVLNGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGSIVQK\r\nNILRDKLTGRPRGVAFVRYNKREEAQEAISALNNVIPEGGSQPLSVRLAEEHGK","organism":"Drosophila melanogaster","uniprot_code":"P19339","uniprot_range_first":123,"uniprot_range_last":294,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MYGNNNPGSNNNNGGYPPYGYNNKSSGGRGFGMSHSLPSGMSRYAFSPQDTEFSFPSSSS\nRRGYNDFPGCGGSGGNGGSANNLGGGNMCHLPPMASNNSLNNLCGLSLGSGGSDDLMNDP\nRASNTNLIVNYLPQDMTDRELYALFRAIGPINTCRIMRDYKTGYSFGYAFVDFTSEMDSQ\nRAIKVLNGITVRNKRLKVSYARPGGESIKDTNLYVTNLPRTITDDQLDTIFGKYGSIVQK\nNILRDKLTGRPRGVAFVRYNKREEAQEAISALNNVIPEGGSQPLSVRLAEEHGKAKAAHF\nMSQMGVVPANVPPPPPQPPAHMAAAFNMMHRGRSIKSQQRFQNSHPYFDAKKFI","mw":19.771,"total_mw":19.771,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM KP, 50 mM NaCl, 10 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.0,"deuteration":null},"purity_method":null,"name":"Wild-type Protein sex-lethal","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL","address":"Heidelberg, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Heidelberg","webpage":"https://www.embl.de/"}],"contributor_name":"Po-chia","contributor_surname":"Chen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-10-16","storage_temperature":10.0,"cell_temperature":25.0,"exposure_time":0.045,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":1.0,"sample_volume":null,"flow_rate":null,"s_min":0.02,"s_max":3.78,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of wild-type Sex-lethal protein in 10 mM KH2PO4, 50 mM NaCl, 10 mM DTT, pH 6 were collected on the P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":16.07,"experimental_mw_error":null,"guinier_i0_mw":16.075,"guinier_i0_mw_error":3.21,"porod_mw":15.3,"porod_mw_error":null,"pddf_i0":1186.0,"pddf_i0_error":8.6,"guinier_i0":1190.6,"guinier_i0_error":6.6,"pddf_rg":1.99,"pddf_rg_error":0.02,"guinier_rg":1.97,"guinier_rg_error":0.1,"pddf_dmax":6.87,"pddf_dmax_error":null,"porod_volume":26.02,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":29,"guinier_point_last":308,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDDX4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}