{"code":"SASDE26","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Solution structures of long-acting insulin analogues and their complexes with albumin.","publication":{"title":"Solution structures of long-acting insulin analogues and their complexes with albumin.","author_list":"Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P","journal":"Acta Crystallogr D Struct Biol","doi":"10.1107/S2059798318017552","pmid":"30950398","published_date":"2019 Mar 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CV","pdb_link":[{"pdb_code":"1bj5","ext_code":"1BJ5","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE26_fit2_model1.pdb","model_mw":133.4,"bead_radius":null,"log":null,"symmetry":"P1","comment":null,"user":329}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/RTTR-1bj5-800_fit_img.png","software":"CRYSOL","chi_square_value":1.741,"p_value":0.0,"fit_residual_plot":"RTTR-1bj5-800_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDE26_fit2.fit","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDE26_fit3_model1_img.png","software":"DAMMIF","pdb_link":[{"pdb_code":"1trz","ext_code":"1TRZ","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE26_fit3_model1.pdb","model_mw":107.0,"bead_radius":2.7,"log":null,"symmetry":null,"comment":null,"user":329}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/HSA_dihex-7_fit_img.png","software":"DAMMIF","chi_square_value":1.665,"p_value":4.3e-05,"fit_residual_plot":"HSA_dihex-7_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDE26_fit3.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SEC–SAXS with flow cell, effective sample path length 1.7 mm, flow rate 0.70 mL/min, 100 µL sample loaded with concentration 10.6 mg/mL.\r\nA Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) was used in combination with an Agilent 1260 Infinity Bio-Inert HPLC/FPLC machine\r\nContinuous 1 s SAXS data-frame measurements of SEC elution.","experiment_description":"Synchrotron SAXS data from solutions of Albumin-insulin degludec (1:12 complex) in 25 mM Na2HPO4, 15.9 mM m-cresol, 15.9 mM phenol, 212.8 mM glycerol, 20 mM NaCl, pH 7.6 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1241 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.50 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":138.5,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":119.6,"porod_mw_error":null,"pddf_i0":0.1017,"pddf_i0_error":null,"guinier_i0":0.102,"guinier_i0_error":null,"pddf_rg":3.782,"pddf_rg_error":null,"guinier_rg":3.8,"guinier_rg_error":null,"pddf_dmax":13.4,"pddf_dmax_error":null,"porod_volume":179.46,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE26_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}