{"code":"SASDE27","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"The structure and stability of the disulfide-linked γS-crystallin dimer provide insight into oxidation products associated with lens cataract formation.","publication":{"title":"The structure and stability of the disulfide-linked γS-crystallin dimer provide insight into oxidation products associated with lens cataract formation.","author_list":"Thorn DC, Grosas AB, Mabbitt PD, Ray NJ, Jackson CJ, Carver JA","journal":"J Mol Biol","doi":"10.1016/j.jmb.2018.12.005","pmid":"30552875","published_date":"2018 Dec 12"},"status":"released","submitted_date":"2018-10-29","released_date":"2018-12-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE27.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE27.txt","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE27_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE27_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE27_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE27_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE27.sascif","experiment":{"instrument":{"detector":{"type":"multiwire","name":"Bruker HI-STAR","resolution":0.0},"name":"Australian Nuclear Science and Technology Organisation/Australian Centre for Neutron Scattering","city":"Sydney","country":"Australia","beamline_name":"Bruker Nanostar II","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Gamma-crystallin S","short_name":null,"sequence":">sp|P22914|CRYGS_HUMAN Gamma-crystallin S OS=Homo sapiens OX=9606 GN=CRYGS PE=1 SV=4\r\nMSKTGTKITFYEDKNFQGRRYDCDCDCADFHTYLSRCNSIKVEGGTWAVYERPNFAGYMY\r\nILPQGEYPEYQRWMGLNDRLSSCRAVHLPSGGQYKIQIFEKGDFSGQMYETTEDCPSIME\r\nQFHMREIHSCKVLEGVWIFYELPNYRGRQYLLDKKEYRKPIDWGAASPAVQSFRRIVE","organism":"Homo sapiens","uniprot_code":"P22914","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSKTGTKITFYEDKNFQGRRYDCDCDCADFHTYLSRCNSIKVEGGTWAVYERPNFAGYMY\nILPQGEYPEYQRWMGLNDRLSSCRAVHLPSGGQYKIQIFEKGDFSGQMYETTEDCPSIME\nQFHMREIHSCKVLEGVWIFYELPNYRGRQYLLDKKEYRKPIDWGAASPAVQSFRRIVE","mw":21.007,"total_mw":21.007,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM sodium phosphate","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.0,"deuteration":null},"purity_method":null,"name":"Gamma-crystallin S monomer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Canberra, Australia","full_name":"Australian National University","webpage":"https://www.anu.edu.au/"}],"contributor_name":"Aidan","contributor_surname":"Grosas","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-23","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":3600.0,"number_of_frames":4,"wavelength":0.1541,"sample_detector_distance":0.73,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.128,"s_max":3.91,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDE27_fit1_model1_img.png","software":"Chimera","pdb_link":[{"pdb_code":"2m3t","ext_code":"2M3T","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE27_fit1_model1.pdb","model_mw":20.93,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":383}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/2m3t-101_fit_img.png","software":"CRYSOL","chi_square_value":1.425,"p_value":0.229414,"fit_residual_plot":"fit4columns1875_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDE27_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS 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One solute concentration of 3.00 mg/ml was measured at 25°C. Four successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":15.8,"experimental_mw_error":null,"guinier_i0_mw":15.8,"guinier_i0_mw_error":3.3,"porod_mw":17.0,"porod_mw_error":null,"pddf_i0":133.5,"pddf_i0_error":0.5,"guinier_i0":131.69,"guinier_i0_error":1.0,"pddf_rg":1.85,"pddf_rg_error":null,"guinier_rg":1.796,"guinier_rg_error":0.03,"pddf_dmax":5.9,"pddf_dmax_error":null,"porod_volume":27.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":11,"guinier_point_last":65,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE27_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}