{"code":"SASDE32","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes","publication":{"title":"Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes","author_list":"Ruiz L, Kaczmarska Z, Gomes T, Aragón E, Torner C, Freier R, Bagiński B, Martin-Malpartida P, de Martin Garrido N, Márquez J, Cordeiro T, Pluta R, Macias M","journal":null,"doi":"10.1101/833319","pmid":null,"published_date":"2019 Nov 07"},"status":"released","submitted_date":"2018-07-24","released_date":"2019-11-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE32.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE32.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE32_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE32_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE32_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE32_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE32.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Mothers against decapentaplegic homolog 5","short_name":"Smad5 MH1","sequence":"GPSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPP","organism":"Homo sapiens","uniprot_code":"Q99717","uniprot_range_first":9,"uniprot_range_last":138,"oligomerization":"unknown","molecular_type":"protein","uniprot_sequence":"MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSSPG\nQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLDICEFPFGSKQKE\nVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQP\nNNTPFPLSPNSPYPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQP\nMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFT\nDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNC\nNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGW\nGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS","mw":15.079,"total_mw":15.079,"number_molecules":0,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Mothers against decapentaplegic homolog 5 - Smad5 MH1","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"IRB","address":"Barcelona, Spain","full_name":"Institute for Research in Biomedicine in Barcelona (IRB)","webpage":"https://www.irbbarcelona.org"}],"contributor_name":"Tiago","contributor_surname":"Lopes","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-02-14","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.09919,"sample_detector_distance":2.87,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.281,"s_max":4.918,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ScÅtter","pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of mothers against decapentaplegic homolog 5 (Smad5 MH1) in 20 mM Tris 150 mM NaCl, pH 7.2 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-to-detector distance of 2.87 m and an X-ray wavelength, λ, of 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. 10 successive 1 s frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The data is recorded from a sample in monomer-dimer equilibrium.","tags":[],"intensity_unit":"arbitrary","experimental_mw":18.55,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":14.11,"pddf_i0_error":0.02,"guinier_i0":15.75,"guinier_i0_error":0.02,"pddf_rg":1.782,"pddf_rg_error":0.04,"guinier_rg":1.853,"guinier_rg_error":0.05,"pddf_dmax":6.6,"pddf_dmax_error":null,"porod_volume":32.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE32_datloglog_img.png","symmetry":null,"last_modified":"2021-02-27T12:29:13.920744+01:00","bragg_peak":[]}