{"code":"SASDE38","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain","publication":{"title":"Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain","author_list":"Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G","journal":"Biophysical Journal","doi":"10.1016/j.bpj.2019.12.025","pmid":null,"published_date":"2020 Jan"},"status":"released","submitted_date":"2018-11-13","released_date":"2019-11-25"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE38.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE38.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE38_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE38_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE38.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Major prion protein","short_name":null,"sequence":"GQGGSHSQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSVMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITVKQHTVQTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGAS","organism":"Ovis 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Cu(II)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"ESRF","address":"Grenoble, France","full_name":"European Synchrotron Radiation Facility","webpage":"http://www.esrf.eu/"}],"contributor_name":"Gabriele","contributor_surname":"Giachin","orcid":"https://orcid.org/0000-0001-7550-0307"}],"concentration_method":null,"concentration_unit":null,"date":"2018-05-04","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":1,"wavelength":0.099,"sample_detector_distance":2.864,"concentration_min":null,"concentration_max":7.0,"sample_volume":null,"flow_rate":null,"s_min":0.103,"s_max":4.928,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDE38_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE38_fit1_model1.pdb","model_mw":16.02,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/logFile_001_1_YmXnJKW.log","symmetry":"P1","comment":null,"user":388}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDE38_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":3.578,"p_value":0.864685,"fit_residual_plot":"SASDE38_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDE38_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_8RO0wJ6.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the C-terminal truncated ovine prion protein (94-234) with VRQ polymorphism in 25 mM ammonium acetate, 250 mM NaCl, 0.001 mM CuSO4, pH 5.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column type, Superdex 200 5/150 GL (GE Healthcare); Flow rate: 0.15 mL/min; Total acquisition time: 25 min; Injection concentration: 7 mg/mL; Injection volume: 50 μL. The data obtained through the sample elution peak (865-905 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":16.1,"experimental_mw_error":null,"guinier_i0_mw":15.5,"guinier_i0_mw_error":null,"porod_mw":15.6,"porod_mw_error":null,"pddf_i0":11.52,"pddf_i0_error":null,"guinier_i0":10.93,"guinier_i0_error":0.03,"pddf_rg":2.417,"pddf_rg_error":null,"guinier_rg":2.15,"guinier_rg_error":0.01,"pddf_dmax":8.7,"pddf_dmax_error":null,"porod_volume":28.8,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":57,"guinier_point_last":113,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE38_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:28:10.454623+02:00","bragg_peak":[]}