{"code":"SASDE49","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.","publication":{"title":"Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.","author_list":"Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X","journal":"Structure","doi":"10.1016/j.str.2019.05.011","pmid":"31257107","published_date":"2019 Aug 6"},"status":"released","submitted_date":"2018-12-11","released_date":"2019-09-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE49.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE49.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE49_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE49_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE49_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE49_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE49.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray 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glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.2,"deuteration":null},"purity_method":null,"name":"Interleukin-1 receptor accessory protein ectodomains with ST2 linker","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Taiwan","full_name":"National Cheng Kung University","webpage":"http://www.ncku.edu.tw/"}],"contributor_name":"Shu-Ying","contributor_surname":"Wang","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-07-24","storage_temperature":null,"cell_temperature":20.0,"exposure_time":3.0,"number_of_frames":600,"wavelength":0.103,"sample_detector_distance":1.5,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.103,"s_max":5.608,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDE49_fit1_model1_img.png","software":"BILBOMD","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE49_fit1_model1.pdb","model_mw":117.9,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":393},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDE49_fit1_model2_img.png","software":"BILBOMD","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDE49_fit1_model2.pdb","model_mw":117.9,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":393}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/multi_state_model_2_1_1_jv00Ew9_fitfoxs_img.png","software":"MES-FoXS","chi_square_value":2.198835,"p_value":0.0,"fit_residual_plot":"multi_state_model_2_1_1_jv00Ew9_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDE49_fit1.dat","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":"SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.","experiment_description":"Synchrotron SAXS data from solutions of Interleukin-1 receptor accessory protein ectodomains with ST2 linker in 10mM HEPES, 150mM NaCl, 3% glycerol, pH 7.2 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 600 successive 3 second frames through the SEC elution profile. Sample-elution peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":52.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":562.0,"pddf_i0_error":0.6048,"guinier_i0":561.3,"guinier_i0_error":1.14,"pddf_rg":3.18,"pddf_rg_error":null,"guinier_rg":3.118,"guinier_rg_error":0.01,"pddf_dmax":10.71,"pddf_dmax_error":null,"porod_volume":76.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":58,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE49_datloglog_img.png","symmetry":null,"last_modified":"2020-11-27T12:01:46.570871+01:00","bragg_peak":[]}