{"code":"SASDE58","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.","publication":{"title":"A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.","author_list":"Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R","journal":"Nat Commun","doi":"10.1038/s41467-019-08865-z","pmid":"30814507","published_date":"2019 Feb 27"},"status":"released","submitted_date":"2018-11-15","released_date":"2019-03-04"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE58.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE58.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE58_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE58_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE58_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE58_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE58.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Putative killer protein, Toxin GraT.","short_name":"GraT","sequence":"MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTEHGPVNVEIVDYENLYFQ","organism":"Pseudomonas putida","uniprot_code":"Q88MI5","uniprot_range_first":1,"uniprot_range_last":91,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNR\nADQHSIRVNDQWRLCFTWTEHGPVNVEIVDYH","mw":11.104,"total_mw":11.104,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"antitoxin GraA, antidote of toxin GraT","short_name":"GraA","sequence":"MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA","organism":"Pseudomonas putida","uniprot_code":"Q88MI6","uniprot_range_first":1,"uniprot_range_last":99,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLS\nICLDTTPEFWLNLQTAFDLRTAEQQHGDEIIGSVQRLVA","mw":10.905,"total_mw":21.81,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Putative killer protein, Toxin GraT","short_name":"GraT.","sequence":"MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTEHGPVNVEIVDYENLYFQ","organism":"Pseudomonas putida","uniprot_code":null,"uniprot_range_first":1,"uniprot_range_last":91,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":11.104,"total_mw":11.104,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris 250 mM NaCl 2 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Complex of toxin GraT with antotoxin GraA","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"VUB","address":"Pleinlaan 2 1050 Brussel ","full_name":"Vrije Universiteit Brussel","webpage":"http://www.vub.ac.be/"}],"contributor_name":"Ariel","contributor_surname":"Talavera","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-06-29","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.5,"number_of_frames":1200,"wavelength":0.10332,"sample_detector_distance":1.999,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.064,"s_max":4.483,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the complex formed between toxin GraT with antotoxin GraA in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 10°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Sample injection concentration: 12.5 mg/mL; Injection volume: 40 μL. 1200 successive 0.500 second frames were collected. The data measured through the SEC elution profile of the sample were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":40.991,"experimental_mw_error":null,"guinier_i0_mw":40.991,"guinier_i0_mw_error":null,"porod_mw":39.0,"porod_mw_error":null,"pddf_i0":0.04879,"pddf_i0_error":1.4e-05,"guinier_i0":0.04886,"guinier_i0_error":3.1e-05,"pddf_rg":2.34,"pddf_rg_error":null,"guinier_rg":2.349,"guinier_rg_error":0.05,"pddf_dmax":7.91,"pddf_dmax_error":null,"porod_volume":63.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":108,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE58_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}